猪细小病毒在PK细胞中的增殖过程

将猪细小病毒南京毒株1（NJ-1）、南京毒株2（NJ-2）和7909毒株接种于PK-15细胞，用免疫荧光检测方法研究了猪细小病毒增殖的基本特性与规律。在PK-15细胞中，3个毒株的增殖规律基本相似，均在感染后12h即可检测到荧光，表明其子代病毒粒子产生，随后逐渐增多，在感染后48h荧光几乎遍布所有细胞，随后荧光开始衰减，在84h时病毒引起细胞大面积崩解，荧光呈现岛屿状分布。通过绘制其一步法生长曲线可知，在病毒感染后12h，细胞培养液中的病毒TCID50／ml。为2．0左右，随后逐渐增高，感染后60h3种毒株的TCID50／mL均达到最高，子代病毒粒子向细胞外释放也达到高峰，其后TCID50逐渐下降。培养液中病毒粒子的丰寿期为7h。

The Replication of Porcine Parvovirus in PK-15 Cell Cultures

Some multiplication properties of three strains of porcine parvovirus(PPV) in porcine kidney cells were studiedby using the immunofluorescence technique.The results indicated that the three strains have the similar characteristics of themultiplication.The virus was observed within cytoplasm at 12 h postinoculation,followed by the highest product at 48 h afterinoculation.Then the viral particles inside host cells began to reduce and caused host cells to crack,releasing from cells.Basedon the results,the one-step growth curve of cells was drawn.It was showed that the virus escaped from host cells at 12 h afterinfection.The viral titers were reached to the highest level at 60 h postinoculation and the viral concentration declined from thenon.