葡萄糖氧化酶基因转化玉米及其后代的抗病性研究

通过花粉管通道法将葡萄糖氧化酶基因(Glucose oxidase,GO)转入玉米自交系,并对转化后代进行了大斑病抗性鉴定。结果表明,授粉后15～20h为最佳外缘基因导入时间段,最适合的转化质粒DNA浓度为100μg·mL-1。转化获得卡那霉素抗性植株244株,PCR阳性植株13株,对转化的D0代PCR阳性植株的抗病性进行了大斑病抗性鉴定,部分转化植株的抗玉米大斑病能力有所提高,其中3株表现高抗。研究为探索大众化玉米转基因和培育抗病玉米自交系提供了新方法。

Glucose oxidase gene transformation of maize and disease resistance analysis

Glucose oxidase gene was introduced into three maize inbred lines by pollen tube pathway, and the D0 generation transformed plants were tested to the maize Exserohilum turcicum resistance. The results showed that DNA concentration and transformation duration obviously influenced the transformation rate. The best transforming condition was DNA concentration 100 μg·mL-1 and to transform at 15-20 h after self-pollination. Total 244 D0 maize plants showed the kanamycin resistance, among which 13 plants showed the positive PCR reaction. The results indicated that D0 PCR-positive plants had improved the ability to resist maize Exserohilum turcicum via field disease resistance test, three D1 offsprings gave the high disease resistance. This study provided a new method to transform maize and cultivate disease resistant maize inbred lines.