Protective effect of magnesium on brain with acute ischemia and reperfusion injury |
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| Authors: | LIU Yu MENG Ran JI Xun-ming NING Ming-ming WANG Xiao-ying |
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| Institution: | 1. Neurology Department, Beijing Shijitan Hospital Affiliated to Capital Medical University, The Ninth Clinical Medical College of Peking University, Beijing 100038, China;
2. Cerebral Vascular Diseases Research Institute, Xuanwu Hospital, Capital Medical University, Key Laboratory of Neurodegenerative Diseases of Ministry of Education, Beijing 100053, China;
3. Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA. |
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| Abstract: | AIM: To dynamically observe the protective effect of magnesium on the brain with ischemia and reperfusion injury in the middle cerebral artery occlusion (MCAO) rat model using the device of laser Doppler flowmetry (LDF) .METHODS: Twenty-four Sprague-Dawley male rats (280-300 g) were used to establish MCAO model with the conventional line-embolism method.The rats were divided into 4 groups according to the initial time of peritoneal injection of magnesium sulfate (MgSO4).The rats in MgSO4 groups were treated with 25% MgSO4 at 160 mg/kg at time points of 20 min, 30 min and 40 min after ischemia.The rats in control group were treated with the same volume of normal saline.The real-time fluctuation of the local cerebral blood flow (CBF) was dynamically monitored by LDF.The degree of nervous functional defect was evaluated by calculating the neurological impairment score of the rats after suffered from 2 h ischemia and followed with 24 h reperfusion.All the rats were killed with an end breaking method, and the volumes of brain infarction were evaluated by TTC staining at the brain slices obtained by autopsy.RESULTS: The fluctuating features in 3 MgSO4 treatment groups were as follows: when reperfusion began, the local CBF appeared and increased smoothly, then approached to its baseline step by step, and kept at the stable level in the whole reperfusion period.The CBF in control group fluctuated sharply in the whole reperfusion period associated with the heart beating, which was significantly different from those in MgSO4 treatment groups.The average volume of brain infarction in MgSO4 treatment groups was 26.5%, 36.5% and 24.5%, respectively, and was 54.0% in control group.The local nervous defect scores in MgSO4 treatment groups were 5.670±1.003, 8.670±1.211 and 7.170±1.472, respectively, and was 11.170±0.983 in control group.CONCLUSION: Vasomotor functional improvement might be one of the protective mechanisms of magnesium on the brain with cerebral ischemia and reperfusion injury in acute ischemic stroke. |
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| Keywords: | Magnesium sulfate Brain Ischemia Reperfusion |
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