Inhibitory effect of propolis on proliferation of K562 cells in vitro |
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Authors: | WANG Ming ZHANG Yao QI Zhi-lin BI Fu-yong |
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Affiliation: | Department of Biochemistry, Wannan Medical College, Wuhu 241002, China |
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Abstract: | AIM: To study the effect of propolis on the proliferation of K562 cells.METHODS: K562 cells were cultured in vitro. Cell proliferation was measured by MTT method. The apoptotic rate was determined by flow cytometry. RT-PCR was applied to detect mRNA expression of Nup98. The protein level of Nup98 was determined by Western blotting. RESULTS: The inhibitory rates of proliferation induced by propolis at the concentrations of 2 mg/L, 20 mg/L and 200 mg/L were obviously higher than that in control cells in a time-and dose-dependent manner. The apoptotic rate was increased in a dose-dependent manner. High concentration of propolis down-regulated the expression of Nup98 at mRNA and protein levels. CONCLUSION: Propolis inhibits the proliferation and induces apoptosis in K562 cells. The mechanism may be related with down-regulation of Nup98. |
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Keywords: | Propolis Leukemia K562 cells Nucleoporin 98 |
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