Microinjection of AT1 and AT2 receptor antagonists into PVN affects renal sympathetic nerve activity in chronic heart failure rats |
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Authors: | PAN Li-hua QIN Xiao-tong ZHU Jian-hua GUI Le |
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Institution: | Department of Cardiology, The Affiliated Hospital, Nantong University, Nantong 226001, China |
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Abstract: | AIM: To examine the renal sympathoexcitation affected by microinjection of angiotensin Ⅱ type 1 (AT1) receptor antagonist L-158809 and angiotensin Ⅱ type 2 (AT2) receptor antagonist PD123319 into paraventricular nucleus (PVN) in heart failure rats.METHODS: Left anterior descending coronary artery ligation was used to induce rat heart failure (HF) . Four weeks after operation, the left ventricular end-diastolic pressure (LVEDP), the ratios of heart weight/body weight and lung weight/body weight, and the ratio of infarct area of the left ventricle were observed. Under anesthesia, SD rats were fixed into the brain stereo controller to locate PVN for microinjection and the artificial cerebrospinal fluid (ACSF) was used for control. The left kidney was exposed by retroperitoneal approach and the renal sympathetic nerve was separated under surgical microscope. The heart rate, blood pressure and the activity of renal sympathetic nerve discharge (RSNA) were recorded by POWERLAB 8/30 system. RESULTS: Microinjection of AT1 receptor antagonist into PVN induced a decrease in RSNA in both HF rats and sham rats. The RSNA responses to L-158809 in the HF rats were significantly greater (P<0.05) than those in the sham rats. However, microinjection of AT2 receptor antagonist and ACSF into PVN induced no change of RSNA in both HF and sham rats. CONCLUSION: There are some differences of sympathetic nerve outputs between using AT1 receptor antagonist and AT2 receptor antagonist on PVN, indicating the up-regulation of AT1 receptors in PVN during HF. The central renin-angiotensin-aldosterone system(RAAS) may be affected by AT1 receptor, not by AT2 receptor. |
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Keywords: | Heart failure Paraventricular nucleus Angiotensin Ⅱ type 1 receptor antagonist Renal sympathetic nerve activity |
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