Antioxidative activity of extracts from Luffa cylindrica |
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| Authors: | PAN Yong-qin LI Jing ZHU Wei-jie HE Hui-juan |
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| Institution: | 1. Department of Pathophysiology, School of Medicine,Jinan University, Guangzhou 510632, China;
2. Institute of Reproductive Immunology, College of Life Science and Technology,Jinan University, Guangzhou 510632, China |
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| Abstract: | AIM: To isolate the active compounds of Luffa cylindrica and investigate their antioxidative activity. METHODS: Antioxidants from Luffa cylindrica were isolated using solvent extraction and Sephadex column chromatography. Active compounds of the extracts were identified with the antioxidative activity assays. RESULTS: With solvent extracting procedure, four extracts (Ⅱ, Ⅲ, Ⅳ and Ⅴ) were obtained. Among these extracts, extract Ⅲ had significant antioxidative activity, which exerted a potent scavenging effect for both DPPH· and OH· assays. For further purification, extract Ⅲ was subject to Sephadex column and five fractions ( A, B, C, D and E ) were isolated. Fraction D and E showed good efficiency on scavenging DPPH· and OH·, and exhibited flavanoid characteristics. CONCLUSION: Luffa cylindrica efficiently scavenges free radical species, and flavanoid exerts this action. |
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| Keywords: | Luffa cylindrica Antioxidation Flavones Free radicals |
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