Effects of marine fungal metabolites 1386A from the South China Sea on proliferation,apoptosis and mitochondrial membrane potential in gastric cancer cell line MCG-803 |
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Authors: | CHEN Chuang-qi FANG Le-kun LIU Jia-wei ZHANG Jian-wei YANG Guang-xin YANG Wan |
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Institution: | 1. Department of Gastrointestinal Surgery, The First Affiliated Hospital;2. Zhongshan Medical School, Sun Yat-sen University, Guangzhou 510080, China. E-mail: ccqsums@gmail.com |
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Abstract: | AIM: To investigate the effects of marine fungal metabolites 1386A from the South China Sea(1386A) on gastric cancer cell line MCG-803.METHODS: The effects of 1386A on cell viability and cell growth were detected by MTT method and colony-forming assay, respectively. The changes of cell cycle, cell apoptosis and mitochondrial membrane potential were determined by flow cytometry.RESULTS: The IC50 of cell viability on MCG-803 cells after treated with 1386A for 24 h, 48 h and 72 h was 29.70 μmol/L, 14.07 μmol/L or 13.47 μmol/L, respectively. The IC50 of the cell growth on MCG-803 cells after treated with 1386A for 48 h was 3.27 μmol/L. After treated with 1386A for 48 h, the MCG-803 cells in S stage was increased from 25.6% to 56.8%. After treated with 1386A for 24 h, the early apoptosis of MCG-803 cells was increased from 3.34% to 8.39%, and the mitochondrial membrane potential of MCG-803 cells was decreased by 56.26%.CONCLUSION: 1386A has an inhibitory effect on the cell growth in MCG-803 cells through the mitochondrial pathway. |
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Keywords: | Marine fungal metabolies 1386A from the South China Sea Stomach neoplasms MGC-803 cells Apoptosis Mitochondrial membrane potentials |
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