Inhibition of 293T cell proliferation by blocking miR-20 and miR-106 expression with antisense RNA |
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| Authors: | LI You-jie ZHANG Shuai GAO Zong-hua ZHANG Wen-juan XIE Shu-yang ZHANG Peng-ju JIANG An-li |
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| Institution: | 1. Institute of Biochemistry and Molecular Biology, Medical College, Shandong University, Jinan 250012, China;2. Institute of Medical Molecular Genetics, Binzhou Medical University, Yantai 264003, China;3. Department of Hematology, Yantaishan Hospital, Yantai 264000, China. E-mail:jianganli@sdu.edu.cn |
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| Abstract: | AIM: To inhibit 293T cell proliferation by reducing miR-20 and miR-106 expression with antisense RNA. METHODS: Antisense RNA (or antisense oligonucleotides, ASO) specific to miR-20 and miR-106 was synthesized, and the 293T cells were treated with these antisense RNA. Then, the cell proliferation derived from suppression of antisense RNA was studied by microscopy and fluorescence-activated cell sorter. The expression of miRNA and its target gene Rb were analyzed by real-time PCR and ELISA, respectively. RESULTS: Our results showed that ASO could inhibit the expression of miR-20 and miR-106 effectively and inhibit 293T cell proliferation. The results also demonstrated that ASO specific to miR-106 could up-regulate anti-oncogene Rb expression. CONCLUSION: ASO could inhibit 293T cell proliferation by inhibiting miRNA expression and up-regulating Rb expression. |
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