Influence of chronic stress on vasoactive substances and inflammatory factor in rat model with acute myocardial infarction by factorial analysis

AIM: To investigate the effect of chronic unpredictable mild stress(CUMS) on plasma endothelin, nitric oxide and interleukin-6, high-sensitivity C-reactive protein(hs-CRP) in rats after acute myocardial infarction(AMI).METHODS: After the rats of myocardial infarction(MI) were established, the animals were treated under the condition of CUMS for 4 weeks, then the contents of plasma(or serum) endothelin, nitric oxide, interleukin-6 and hs-CRP were measured and data were analyzed by two factor factorial analysis. RESULTS: The results of factorial analysis showed: MI alone had no significantly effect on the level of nitric oxide(P>0.05), but CUMS had significantly effect on increasing the level of nitric oxide(P<0.01). The CUMS had significantly interaction with MI on increasing the level of nitric oxide(P<0.01). Both MI and CUMS increased the level of hs-CRP(all P<0.01). However, no interaction was discovered between MI and CUMS(P>0.05). Both MI and CUMS had no effect on the level of ET-1 and IL-6(P>0.05).CONCLUSION: CUMS increases nitric oxide content and has cooperative effect with MI on increasing NO, both MI and CUMS significantly increase the level of CRP, but have no effect on the level of ET-1 or IL-6, suggesting that the abnormal increase in nitric oxide and hs-CRP contents may be the important pathophysiological changes of post-MI depression.