Severe hypertriglyceridemia appears in apolipoprotein CⅢ transgenic mouse

AIM: To investigate the biological functions of human apolipoprotein CⅢ (hApoCⅢ) on metabo-lic regulation, hApoCⅢ transgenic mouse model was established.METHODS: hApoCⅢ transgenic mice were generated by injecting hApoCⅢ genomic fragment into fertilized mouse oocytes, and transgenic line was screened using PCR and Southern blotting. Plasma lipid analysis, fast protein liquid chromatography(FPLC), oral fat load test, post-heparin plasma lipoprotein lipase(LPL) activity analysis and glucose tolerance test were performed to characterize the phenotypic changes of lipid and glucose metabolism in the transgenic mice.RESULTS: We successfully generated the hApoCⅢ transgenic mouse model, which showed evident hypertriglyceridemia(HTG) , delayed triglyceride(TG) clearance and reduced LPL activity in post-heparin plasma while no change was detected in glucose tolerance test.CONCLUSION: It is suggested that the manifested evident HTG in hApoCⅢ transgenic mice may be due to inhibition of LPL activity in post-heparin plasma, which would lead to a prolonged clearance of plasma TG.