Effect of all-trans-retinoic acid on the proliferation and expression of α-SMA in human embryonic lung fibroblasts |
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Authors: | LI Jia-xin HAI Guang-fan JIA Yan-long XIA Wu CHEN Yong-feng LIU Ju-yuan |
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Affiliation: | 1. Department of Pharmacology,Xinxiang Medical University, Xinxiang 453003, China;2. Department of Respiratory Medicine, Third Affiliated Hospital, Xinxiang Medical University, Xinxiang 453003, China |
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Abstract: | AIM: To investigate the effects of all-trans-retinoic acid (ATRA) on the proliferation and differentiation of transforming growth factor β1(TGF-β1)-stimulated human embryonic lung fibroblasts (HFL-I).METHODS: The HFL-I cells were cultured in vitro and were pretreated with ATRA for 3 days at the concentrations of 0.1 μmol/L, 1 μmol/L and 10 μmol/L. The proliferation of HFL-1 cells was detected by MTT method. The mRNA expression of α-smooth muscle actin(α-SMA) in HFL-I cells stimulated with TGF-β1 for 0 h, 6 h, 12 h, 24 h, 48 h and 72 h was detected by RT-PCR and the protein expression of α-SMA at the time points of 1,3 and 5 days was detected by Western blotting. The mRNA expression of α-SMA in HFL-I cells pretreated with different concentrations of ATRA for 24 h was detected the by RT-PCR and the protein expression at time point of 3rd day was detected by Western blotting. RESULTS: Different concentration of ATRA inhibited the proliferation of HFL-I in a dose-dependent manner (P<0.05). Both mRNA and protein expression of α-SMA in HFL-I cells pretreated with TGF-β1 was up-regulated (P<0.05). ATRA down-regulated the mRNA and protein expression of α-SMA induced by TGF-β1 in a dose-dependent manner (P<0.05). CONCLUSION: ATRA inhibits the proliferation and TGF-β1-stimulated differentiation in HFL-I cells by down-regulating the mRNA and protein expression of α-SMA. |
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Keywords: | Pulmonary fibrosis Myofibroblast Transforming growth factor beta All-trans-retinoic acid |
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