Role of calcium-sensing receptor in hypoxia-induced proliferation of rat pulmonary artery smooth muscle cells |
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| Authors: | LI Guang-wei XING Wen-jing HAO Jing-hui BAI Shu-zhi LI Hong-xia LIN Yan XU Chang-qing |
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| Affiliation: | 1. Department of Pathophysiology, Qiqihar Medical University, Qiqihar 161006, China;2. Department of Pathophysiology, Harbin Medical University, Harbin 150086, China;3. Bio-pharmaceutical Key Laboratory of Heilongjiang Province, Harbin 150086, China |
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| Abstract: | AIM: To explore the role of calcium-sensing receptor (CaSR) in rat pulmonary artery smooth muscle cells (PASMCs) and its effect on hypoxia-induced proliferation of PASMCs. METHODS: The expression and distribution of CaSRs were detected by Western blotting and immunofluorescence observation. The intracellular concentration of free calcium ([Ca]i) was determined by confocal laser scanning microscopy. The cell viability was analyzed by MTT assay. The expression of PCNA and CaSRs was determined by Western blotting. RESULTS: CaSR protein was expressed in rat PASMCs. Hypoxia significantly increased the expression of CaSR and PCNA,[Ca]i and the cell viability. GdCl3 (an agonist of CaSR) or NPS2390 (an antagonist of CaSR) amplified or weakened the effect of hypoxia, respectively.CONCLUSION: CaSR is expressed in rat PASMCs. The activation of CaSR is involved in the proliferation of PASMCs induced by hypoxia. |
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| Keywords: | Receptors calcium-sensing Pulmonary arterial smooth muscle cell Hypoxia Cell proliferation |
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