Role of caveolin-1 on down-regulation of LPS-induced monocyte chemotactic protein 1 by 17β-estradiol in vascular smooth muscle cells |
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| Authors: | JIANG Ping Polat·Tuersun LI Jun-hong Xuehereti·Enayeti NIE Yong-mei Aynur·Galilee ZHANG Shun-jie SHEN Yue-liang |
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| Institution: | 1. Department of Physiology, School of Basic Medicine, Xinjiang Medical University, Urumqi 830011, China;
2. Department of Pathophysiology, Zhejiang University School of Medicine, Hangzhou 310058, China |
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| Abstract: | AIM: To elucidate the effect of caveolin-1 on the down-regulation of LPS-induced monocyte chemotactic protein 1 (MCP-1) by 17β-estradiol (E2) in vascular smooth muscle cells (VSMCs).METHODS: The primary-cultured VSMCs were exposed to E2 at concentrations of 10-9-10-6 mol/L. LPS-induced MCP-1 production was assayed by ELISA. The protein expression of caveolin-1 was determined by Western blotting and was silenced by β-methyl cyclodextrin(β-MCD) or caveolin-1 specific siRNA. RESULTS: LPS significantly enhanced MCP-1 production. E2 at concentrations of 10-9-10-6 mol/L inhibited LPS-induced MCP-1 production. The use of caveolin-1 inhibitor β-MCD or silencing the protein expression of caveolin-1 by specific siRNA largely impaired LPS-enhanced MCP-1 production, while E2 markedly inhibited caveolin-1 expression. CONCLUSION: Inhibition of LPS-induced MCP-1 production by E2 is related to the suppression of caveolin-1. |
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| Keywords: | Estradiol Lipopolysaccharides Monocyte chemotactic protein-1 Caveolin-1 Vascular smooth muscle cells |
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