Effect of drug-containing serum of Liuwei Dihuang pills on TGF-β/Smad signaling pathway in HK-2 cells |
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| Authors: | HAO Yan-peng ZHANG Yue LIU Yu-min LU Min WANG Fei LU Hai-ying |
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| Affiliation: | 1. Experiment Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;2. College of Nursing, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China |
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| Abstract: | AIM: To investigate the effect of the drug-containing serum of Liuwei Dihuang pills on the TGF-β1/Smad signaling pathway in HK-2 cells.METHODS: The proliferation of HK-2 cell was detected by MTT method. Western blotting analysis was used to investigate the effect of the drug-containing serum of Liuwei Dihuang pills on the protein expression of the molecules of Smad signal transduction pathway.RESULTS: The drug-containing serum of Liuwei Dihuang pills promoted the proliferation of HK-2 cells. The level of Smad2 phosphorylation in HK-2 cells treated with 10% drug-containing serum of Liuwei Dihuang pills was significantly lower than that in the cells treated with TGF-β1. Furthermore, SnoN, a negative factor in Smad signaling pathway, was up-regulated in HK-2 cells treated with 10% drug-containing serum.CONCLUSION: Drug-containing serum of Liuwei Dihuang pills inhibits TGF-β1/Smad signaling pathway, including reducing Smad2 phosphorylation and promoting SnoN protein expression. |
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| Keywords: | Liuwei Dihuang pills Renal tubular epithelial cells Signal transduction Protein expression |
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