| Abstract: | AIM: To study the inhibitory effect of Naja naja venom components on the proliferation of human glioma U251 cells.METHODS: MTT assay was used to compare the inhibitory effect of various kinds of Naja naja venom components on human glioma U251 cells and the efficient components were selected. Flow cytometry was performed to detect apoptotic rate and cell cycle. The morphological changes of human glioma U251 cells were observed under laser scanning confocal microscope and transmission electron microscope.RESULTS: Eleven components of Naja naja venom were tested. The growth-inhibiting effects of them on U251 cells were observed, among which the component KD II-3 showed the inhibitory effect on U251 cells in a dose- and time-dependent manner. After treated with KD II-3 at the concentrations of 1 mg/L, 3.75 mg/L, 7.5 mg/L and 15 mg/L, the apoptotic rates of U251 cells were 13.3%, 17.7%, 20.0% and 22.4%, respectively. U251 cells were blocked in G0/G1 phase and the peak of sub-G1 phase appeared. Under laser scanning confocal microscope, U251 cells showed shrinkage of the cell membrane, chromatin condensation and fragmentation after treated with KD II-3 at the concentration of 7.5 mg/L for 24 h, and karyopyknosis and chromatin condensation in U251 cells were found under transmission electron microscope.CONCLUSION: Component KD II-3 of Naja naja venom inhibits the proliferation of U251 cells and promotes apoptosis. |
