Cholesterol depletion up-regulates expression of prohibitin in human prostate carcinoma PC-3 cells |
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Authors: | DONG Pei LI Yong-hong YAO Kai LIU Zhuo-wei ZHOU Fang-jian |
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Institution: | State Key Laboratory of Oncology in Southern China, Department of Urology, Cancer Center, Sun Yat-sen University, Guangzhou 510060, China |
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Abstract: | AIM: To study how cholesterol delpetion affects prohibitin expression and the development of prostate cancer. METHODS: Human prostate carcinoma PC-3 cells were cultured in normal medium (NM) and cholesterol delpetion medium (CDM) for 48 h.The mRNA expression of prohibitin was detected by real-time PCR. Prohibitin promoter was cloned and inserted into PGL3-Basic to reconstruct plasmid. The plasmid was transiently transfected into PC-3 cells. The cells were cultured in the medium with different concentrations of cholesterol for 48 h and luciferase expression was detected.RESULTS: The results of real-time PCR showed that the mRNA level of prohibitin increased about 19 times in PC-3 cells in the presence of CDM. After transfected with prohibitin promoter plasmid for 48 h, PC-3 cells cultured in CDM had higher luciferase expression than the cells cultured in NM or in CDM with cholesterol. CONCLUSION: Cholesterol depletion up-regulates prohibitin expression in PC-3 cells, which may be one of the self-prophylactic and regulatory mechanisms to protect PC-3 cells from apoptosis caused by cholesterol insufficiency. |
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Keywords: | Prostatic neoplasms Cholesterol Prohibitin Promoter |
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