Indomethacin induces gastric cancer cell apoptosis through Akt/GSK3β/NAG-1 pathway |
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Authors: | PANG Rui-ping HU Pin-jin ZENG Zhi-rong CHEN Wei |
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Affiliation: | 1. Department of Physiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China;2. Department of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China |
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Abstract: | AIM: To investigate whether indomethacin induces gastric cancer cell apoptosis through Akt/GSK3β/NAG-1 pathway.METHODS: Gastric cancer cell line MGC-803 was used in the study. Cell viability was measured by MTT method. Hoechst 33258 nuclear staining and flow cytometry analysis were used to determine apoptosis. The protein expression level was examined by Western blotting. RESULTS: Indomethacin induced MGC-803 cell apoptosis via caspase-dependent pathway. Indomethacin inhibited Ser473-Akt and Ser9-GSK3β phosphorylation and up-regulated the expression of non-steroidal anti-inflammatory drug-activated gene-1 (NAG-1). Inhibition of PI3K or Akt alone also increased NAG-1 expression. Moreover, the effect of indomethacin on NAG-1 expression was abolished by pretreatment of the cells with GSK3β inhibitor SB216763. CONCLUSION: Indomethacin induces gastric cancer cell apoptosis through Akt/GSK3β/NAG-1 pathway. |
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Keywords: | Indomethacin Cyclooxygenase MGC-803 cells Apoptosis Akt/GSK3β/NAG-1 pathway |
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