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| 奶牛源MRSA菌株的分离鉴定和耐药性分析 | |
| 引用本文： | 冯清,林伟东,张大帅,戴迪,廖俊,王仙,汪怿旸,梁祥焕,张军,秦爱建,金文杰. 奶牛源MRSA菌株的分离鉴定和耐药性分析[J]. 中国畜牧兽医, 2016, 43(7): 1862-1868. DOI: 10.16431/j.cnki.1671-7236.2016.07.031 |
| 作者姓名： | 冯清 林伟东 张大帅 戴迪 廖俊 王仙 汪怿旸 梁祥焕 张军 秦爱建 金文杰 |
| 作者单位： | 1. 扬州大学兽医学院, 江苏省动物预防医学重点实验室, 江苏省动物重要疫病与 人兽共患病防控协同创新中心, 扬州 225009; 2. 扬州大学实验农牧场, 扬州 225009 |
| 基金项目： | 江苏省"青蓝工程"；扬州市社会发展科技攻关项目（YZ2011091）；江苏省农业科技自主创新资金项目（CX（14）2088）；江苏高校优势学科建设工程（PAPD）；常州市科技支撑项目（CE20152006） |
| 摘 要： | 本研究对部分奶牛场采集的502份隐性乳房炎试验（california mastitis test，CMT）检测呈阳性的奶样进行细菌分离、鉴定，运用多重PCR方法筛选出耐甲氧西林金黄色葡萄球菌（methicillin-resistant Staphylococcus aureus，MRSA），并对分离出的MRSA用纸片扩散法进行药敏试验，检测其对19种抗菌药物的敏感性。结果显示，分离到细菌的奶样有452份，其中检出葡萄球菌338株，MRSA 89株；MRSA分离株主要对万古霉素、磷霉素、氯霉素和克拉霉素敏感，对青霉素、多黏菌素、大观霉素及头孢他啶具有较强的耐药性。本研究为指导奶牛乳房炎用药、MRSA菌株的快速鉴定及制定MRSA感染的防控措施提供参考。 |
| 关 键 词： | 奶牛乳房炎 耐甲氧西林金黄色葡萄球菌 多重PCR 药敏 |
Isolation,Indentification and Antibiotic Resistance Analysis of Methicillin-resistant Staphylococcus aureus (MRSA) from Raw Cow Milk |
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| FENG Qing,LIN Wei-dong,ZHANG Da-shuai,DAI Di,LIAO Jun,WANG Xian,WANG Yi-yang,LIANG Xiang-huan,ZHANG Jun,QIN Ai-jian,JIN Wen-jie. Isolation,Indentification and Antibiotic Resistance Analysis of Methicillin-resistant Staphylococcus aureus (MRSA) from Raw Cow Milk[J]. China Animal Husbandry & Veterinary Medicine, 2016, 43(7): 1862-1868. DOI: 10.16431/j.cnki.1671-7236.2016.07.031 | |
| Authors: | FENG Qing LIN Wei-dong ZHANG Da-shuai DAI Di LIAO Jun WANG Xian WANG Yi-yang LIANG Xiang-huan ZHANG Jun QIN Ai-jian JIN Wen-jie |
| Affiliation: | 1. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Ministry of Education Key Laboratory of Poultry Preventive Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China; 2. Yangzhou Experiment Dairy Farm, Yangzhou University, Yangzhou 225009, China |
| Abstract: | In this study,we collected 502 california mastitis test (CMT) positive milk samples from several cattle farms and then the bacteria were isolated and identified from them.After that,we used multiplex-PCR for screening out methicillin-resistant Staphylococcus aureus (MRSA).Then we investigated the MRSA's antibiotics susceptibility to 19 kinds of antibiotics by discdiffusion method.The results showed that 338 strains of Staphylococci including 89 strains of MRSA were detected out from the 452 milk samples with bacteria.Additionally,most MRSA showed high sensitivity to vancomycin,fosfomycin,chloramphenicol and clarithromycin,while resistant to penicillin,polymyxin,spectinomycin and ceftazidime.The results provided certain factual basis and method for guiding the use of antibiotics in bovine mastitis,rapid identification of MRSA strains and prevention or control of MRSA infection. |
| Keywords: | bovine mastitis MRSA multiplex-PCR drug sensitivity |
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