微波加热快速提取芽孢杆菌与乳酸菌DNA

试验旨在研究快速、简单、高效提取芽孢杆菌与乳酸菌DNA的方法。在微波炉额定功率800 W下，对芽孢杆菌和乳酸菌进行微波处理40~150 s，离心获取上清，收获细菌DNA，将样本DNA PCR扩增后进行琼脂糖凝胶电泳验证，测定其纯度和产量后测序。结果显示，在微波炉功率800 W条件下加热40~150 s均可有效提取芽孢杆菌与乳酸菌DNA。所得DNA质量较好（D_{260 nm}/D_{280 nm}在1.8~2.1之间）。DNA浓度符合PCR检测要求，目的条带清晰，所得测序结果满足常规分子生物学研究。该微波提取方法具有简单、快速、高效的特点，且具有广泛的实用性，为乳酸菌与芽孢杆菌快速分子检测提供了简便手段。

Rapid Extraction of Bacillus and Lactobacillus DNA by Microwave Heating

The assay was aimed to investigate a fast,simple and efficient method with microwave heating for extracting DNA of Bacillus and Lactobacillus.Treated in a microwave oven at rated power (800 W) for a certain time (40 to 150 s),then centrifuged for supernatant.The supernatant was transferred to a sterile tube for PCR testing and agarose gel electrophoresis.Sequencing was carried out after determining the purity and yield.The results showed that heating for 40 to 150 s at 800 W microwave power conditions could effectively extract DNA of Bacillus and Lactobacillus.The obtained DNA quality was good (D_{260 nm}/D_{280 nm} was 1.8 to 2.1).The DNA concentration in line with the PCR testing requirements;Sequencing results was satisfied with the routine molecular biological research.The microwave extraction method had simple,fast and efficient features,and a wide range of practical,which provided a simple means for rapid molecular assay.