副鸡禽杆菌发酵培养工艺研究

本试验从接种方式、培养时间、初始接种量等方面对副鸡禽杆菌C-Apg-8(A型)的发酵培养条件进行了工艺改进。结果显示,通过将现有副鸡禽杆菌生产的二步接种法(一级种子→二级种子(7500mL)→300L)改为四步接种法(4mL→160mL→1600mL→30000mL→300L),接种比例由之前的2.5%变为2.5%~10%,培养方式由大瓶培养变为发酵罐培养,同时在不改变单批次发酵总体积、不增加人员和原材料成本的情况下,最终可以使副鸡禽杆菌发酵单批产量增加约5倍。

Study on Fermentation Cultivation Process of Avibacterium paragallinarum

The fermentation cultivation process of Avibacterium paragallinarum C-Apg-8(serovar A) had been improved in terms of inoculation method, culture time, the initial inoculation quantity of microorganism and so on.The results showed that the single-batch production capacity of Avibacterium paragallinarum fermentation could increase almost five times by changing the present two-step inoculation method(the primary seed→the secondary seed(7500 mL) →300 L) to the four-step inoculation method(4 mL→160 mL→1600 mL→30000 mL→300 L), and adjusting the inoculation proportion from 2.5% to 2.5%-10%.The improved cultivation way had been changed from spinner cultivation to fermenter cultivation without changing the volume of fermentation, and without adding the staff and the cost of raw material.