黄花补血草总黄酮亚急性毒性试验

试验旨在评价黄花补血草总黄酮的安全性，为今后系统研究其药理作用和作为临床安全用药提供试验依据。选取80只1日龄、体重接近的大白鼠，随机分为2大组（Ⅰ、Ⅱ组），其中每大组分成黄花补血草总黄酮高、中、低剂量组和空白对照组，药物组按照15、10、5 g/kg的剂量灌胃给药，对照组不给药，在给药10（Ⅰ组）、21 d（Ⅱ组）后采血测定试验指标。结果显示，Ⅰ组中、低剂量组的大白鼠体增重与对照组相比差异不显著（P>0.05），而高剂量组体增重显著低于对照组（P<0.05）。Ⅱ组低剂量组的大白鼠体增重与对照组相比差异不显著（P>0.05），而中、高剂量组体增重显著低于对照组（P<0.05）；试验组大白鼠的各脏器系数与对照组差异不显著（P>0.05）；在病理解剖过程中发现，随着剂量组浓度的增大，黄花补血草总黄酮对主要器官有一定的病理性损伤，21 d中、高剂量组的肺部组织出现白色肿块，肝脏出现红色出血点。结果表明，黄花补血草总黄酮毒性低，可以安全用于动物，但其剂量不宜过高。

Subacute Toxicity Test of the Total Flavonoids of Limonium aureum (L.)Hill.

To evaluate the safety of the total flavonoids of Limonium aureum (L.) Hill.and lay an experimental basis to study the pharmacological effects and clinical safety in the future,80 Wistarrats with same age and body weight were randomly divided into two groups (group Ⅰ,Ⅱ),and every group was divided into the high dose group (HG),medium dose group (MG),low dose group (LG) and control group (CG),respectively,of which the mice were given to the total flavonoids of Limonium aureum (L.) Hill.by gavage at the doses of 15,10 and 5 g/kg while the mice of CG were not given any medicine.The blood sample were collected and experimental indexes were measured at 10 (group Ⅰ) and 21 d (group Ⅱ).The results showed that comparing with CG,the weight gain of rats in MG and LG of group Ⅰhad no significant difference (P>0.05),and that of HG were significantly decreased (P<0.05);The weight gain of rats in LG of group Ⅱ had no significant difference (P>0.05),and that of MG and HG were significantly decreased (P<0.05);Organ indexes of experimental groups had no significant difference with CG (P>0.05);With increasing concentration of the total flavonoids of Limonium aureum (L.) Hill.,there was pathological damage to major organs and white lumps in lung and red bleeder existed in liver of rats in HG when given total flavonoids of Limonium aureum (L.) Hill.for 21 d.The results indicated that total flavonoids of Limonium aureum (L.) Hill.was low toxicity and could be safely used for animals with minding the dose was not too high.