基于芯片数据和整合分析研究奶牛乳房炎的关键通路

奶牛乳房炎是奶牛的常见病和多发病之一,给奶牛业带来巨大的经济损失,严重制约着奶牛业的发展。为了进一步了解乳房炎的调控机理,对已发表的5套大肠杆菌感染奶牛乳腺上皮细胞的表达谱芯片数据统一进行预处理,并整合大肠杆菌感染1、6、24 h的基因富集分析,以期得到更可靠的影响奶牛乳房炎的关键基因和通路。结果发现,感染1、6和24 h数据集的分析结果中共同下调通路分别为9、30和17个,分析结果确定了一些奶牛乳房炎相关的通路及基因,为进一步揭示其调控机理提供参考。

Study on the Critical Pathways of Dairy Cow Mastitis Based on Microarray Dataset and Integration Analysis

Dairy cow mastitis was one of the common diseases and frequently-occurring diseases, caused huge economic losses, and also severely restricted the development of dairy industry.To further understand the regulation mechanism of mastitis, published five sets of related microarray data sets of cow mammary epithelial cells infected by Escherichia coli were collected, preprocessed with the standardized method, and integrat the result of gene set enrichment analysis with the infection time of 1, 6 and 24 h, so as to get more reliable key genes and pathways which could affect dairy cow mastitis.We find 9 down-regulated pathways common in the infected 1 h data sets, 30 down-regulated pathways common in the infected 6 h data sets, 17 down-regulated pathways common in the infected 24 h data sets.After analysis, we identified a number of dairy cow mastitis related pathways and genes, which would provide a reference for further study of regulation mechanisms.