Nsp9基因在Marc-145细胞上对猪繁殖与呼吸综合征病毒复制的影响

试验旨在验证Nsp9基因是否影响猪繁殖与呼吸综合征病毒（porcine reproductive and respiratory syndrome virus，PRRSV）的复制。构建含有Nsp9全长基因的质粒pIRES2-EGFP-Nsp9，并转染到Marc-145细胞中，接毒之后分别通过实时荧光定量PCR和Western blotting方法测定PRRSV N蛋白在mRNA和蛋白水平的表达。结果显示，转染Nsp9基因的Marc-145细胞上PRRSV的N蛋白在mRNA水平上显著高于未转染Nsp9基因的对照组（P<0.05），是对照组的1.5倍，同时转染Nsp9基因后的Marc-145上PRRSV N蛋白水平也明显高于对照组。随着剂量的增加，N蛋白的表达量在mRNA和蛋白水平都有所增加。由以上结果初步可知，Nsp9基因可以在Marc-145细胞上促进PRRSV的复制，Nsp9基因与其复制密切相关。

Effect of Nsp9 Gene on Replication of PRRSV in Marc-145 Cells

In order to evaluate whether Nsp9 could enhance the replication of porcine reproductive and respiratory syndrome virus (PRRSV), pIRES2-EGFP-Nsp9 plasmid containing whole Nsp9 genome were transfected into Marc-145 cells,Real-time PCR and Western blotting were used to evaluate the expression of N protein after PRRSV was inoculated. The results showed that the level of N protein in the Nsp9 transfected cells was significantly higher than control group (P<0.05),which was 1.5 times higher. Meanwhile,the expression at protein level had the same trend as the mRNA level. When the dose of plasmid was increasing,the expression of N protein both at the mRNA and protein levels were increased. In conclusion,Nsp9 gene could enhance the replication of PRRSV in Marc-145 cells.