马鹿Zfx/Zfy基因的克隆及序列分析

根据GenBank中公布的牛Zfx/Zfy基因的mRNA序列，设计特异性引物，对马鹿Zfx/Zfy基因进行扩增，并对该序列进行分析。结果表明，克隆得到马鹿Zfx基因序列长为2 115 bp，GenBank登录号为KP257294.1，编码696个氨基酸；Zfy基因序列长为2 406 bp，GenBank登录号为KU041539，编码801个氨基酸。依据Zfx/Zfy基因氨基酸序列构建进化树，分析表明，马鹿Zfx基因与人的亲缘关系较近，与聚为一类的野牛、藏羚羊、虎、家猫、家牛、家犬、绵羊的亲缘关系稍远；Zfy基因与家牛、野牛、藏羚羊、绵羊的亲缘关系较近，与其他动物及人的亲缘关系较远。本研究首次克隆了马鹿Zfx/Zfy基因，为研究该基因蛋白质的结构和功能奠定了基础。

Cloning and Sequence Analysis of Zfx/Zfy Gene in Cervus elaphus

Specific primers were designed according to the mRNA sequence of Bos taurus Zfx/Zfy genes provided in GenBank.Zfx/Zfy sequences of Cervus elaphus were cloned and analyzed.The results showed that the cloned Zfx gene sequence of Cervus elaphus was 2 115 bp (GenBank accession No:KP257294.1) that encoded 696 amino acids,the cloned Zfy gene sequence was 2 406 bp (GenBank accession No:KU041539) that encoded 801 amino acids.The phylogenetic trees of Zfx and Zfy amino acid were obtained using the Neighbor-Joining method of DNAStar software.Analysis showed that Zfx of Cervus elaphus was closely related to Homo sapiens,compared with Bison,Pantholops hodgosoniiand,Panthera tigris,Felis catus,Bos taurus,Canis lupus familiaris,Ovis aries.Zfy of Cervus elaphus was closely related to Bos taurus,Bison,Ovis aries,Pantholops hodgosonii compared with others.Zfx/Zfy genes of Cervus elaphus were cloned and sequenced for the first time,and this research provided a foundation for further studying the structure and biological functions of the proteins.