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| 犬瘟热病毒V蛋白的表达、鉴定及亚细胞定位 | |
| 引用本文： | 刘莉,郝福星,袁维峰,封家旺. 犬瘟热病毒V蛋白的表达、鉴定及亚细胞定位[J]. 中国畜牧兽医, 2016, 43(7): 1723-1728. DOI: 10.16431/j.cnki.1671-7236.2016.07.010 |
| 作者姓名： | 刘莉 郝福星 袁维峰 封家旺 |
| 作者单位： | 1. 江苏农牧科技职业学院, 泰州 225300; 2. 中国农业科学院北京畜牧兽医研究所, 北京 100193; 3. 西藏职业技术学院, 拉萨 850030 |
| 基金项目： | 公益性行业（农业）科研专项（201303042） |
| 摘 要： | 为研究犬瘟热病毒（canine distemper virus，CDV） V蛋白的功能，将CDV V基因片段与pGEX-6P-1载体连接，构建pGEX-6P-1-CDV-V重组表达质粒，通过原核表达系统表达了V蛋白，并将纯化的V蛋白免疫BALB/c小鼠，制备阳性血清。同时，将CDV V基因片段与pcDNA3.1载体连接，构建pcDNA3.1-CDV-V重组表达质粒，经转染Vero细胞后，用激光共聚焦技术确定V蛋白在真核细胞中的分布及亚细胞定位。结果显示，成功表达了V蛋白，并制备了阳性血清。重组质粒pcDNA3.1-CDV-V在外源真核细胞Vero中获得了表达，表达蛋白主要聚集于细胞质。本试验结果为进行CDV V蛋白的功能研究奠定了基础。 |
| 关 键 词： | 犬瘟热病毒 V蛋白 原核细胞 亚细胞定位 |
Expression,Identification and Subcellular Localization of V Protein of Canine Distemper Virus |
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| LIU Li,HAO Fu-xing,YUAN Wei-feng,FENG Jia-wang. Expression,Identification and Subcellular Localization of V Protein of Canine Distemper Virus[J]. China Animal Husbandry & Veterinary Medicine, 2016, 43(7): 1723-1728. DOI: 10.16431/j.cnki.1671-7236.2016.07.010 | |
| Authors: | LIU Li HAO Fu-xing YUAN Wei-feng FENG Jia-wang |
| Affiliation: | 1. Jiangsu Animal Husbandry and Veterinary College, Taizhou 225300, China; 2. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 3. Tibet Vocational Technical College, Lhasa 850030, China |
| Abstract: | In order to study the function of canine distemper virus (CDV) V protein,V gene fragment was inserted into the pGEX-6p-1 vector,and then pGEX-6p-1-CDV-V recombinant expression plasmid was obtained.Purified V protein immunized BALB/c mice to prepare the positive serum.Meanwhile,to confirm the distribution of V protein and subcellular localization with confocal laser technology,a eukaryotic expression recombinant plasmid pcDNA3.1-CDV-V was built,then transfected into Vero cells.The results showed that V protein was successfully expressed and the positive serum was prepared.The recombinant plasmid pcDNA3.1-CDV-V was expressed in Vero cells and mainly expressed in cytoplasm.The results laid a function for functional studies of CDV V protein. |
| Keywords: | canine distemper virus V protein prokaryotic cell subcellular localization |
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