亚洲黑熊(Ursusthibetanus)血型检测

本试验旨在分析亚洲黑熊（Ursusthibetanus）是否具有不同血型。采集中国福建省的40只亚洲黑熊全血样本，使用试管法检测黑熊的血型抗原及血型抗体，抗体的同种型使用2-巯基乙醇鉴定，并对黑熊血浆进行质谱分析。4只黑熊的血浆中检出抗体，该抗体可能是IgM型，可凝集30只亚洲黑熊的红细胞。10只亚洲黑熊血液使用人类血型试剂检测，4只黑熊具有类似人类O型血型，6只具有类似人类B型血型。质谱结果显示，黑熊血清蛋白与大熊猫（Ailuropodamelanoleuca）血清类白蛋白同型异构体1（serum albumin-like isoform 1）有很高同源性。上述结果提示，亚洲黑熊具有至少1个血型系统，该系统至少具有2种血型。如果患病黑熊需要接受输血治疗，交叉配血试验是必需的。此外，在紧急情况下，大熊猫可接受亚洲黑熊血液。

Blood Typing of Asiatic Black Bear (Ursusthibetanus)

In this study,we aimed to determine whether bear had different blood group systems.Whole blood samples were collected from 40 Asiatic Black bears in Fujian province,China.Tube method was used to detect antibodies in plasma,and antibody isotype was determined with 2-mercaptoethanol.Plasma was further analyzed by mass spectrometry.The plasma from four bears had antibodies,possibly IgM isoform,which could agglutinate RBCs from 30 bears.Blood samples from 10 bears were tested by human blood typing reagents.The results showed that 4 bears had blood type like human type O,while 6 bears had like human type B.Plasma protein had extensive homology to serum albumin-like isoform 1 found in giant panda (Ailuropodamelanoleuca).We suggested that Asiatic Black bear might have at least one blood group system with two blood types.If the sick bear needs blood transfusion,a cross-matching test was necessary.Moreover,giant panda might receive blood from Asiatic Black bear in case of emergency.