首页 | 本学科首页   官方微博 | 高级检索  
     

黄藤素对山羊子宫内膜上皮细胞的体外毒性研究
引用本文:闫宝琪,董书伟,王东升,那立冬,桑梦琪,杨洪早,张世栋,严作廷. 黄藤素对山羊子宫内膜上皮细胞的体外毒性研究[J]. 中国畜牧兽医, 2016, 43(12): 3239-3244. DOI: 10.16431/j.cnki.1671-7236.2016.12.023
作者姓名:闫宝琪  董书伟  王东升  那立冬  桑梦琪  杨洪早  张世栋  严作廷
作者单位:1. 中国农业科学院兰州畜牧与兽药研究所, 中国农业科学院临床兽医学研究中心, 甘肃省中兽药工程技术研究中心, 兰州 730050;
2. 甘肃农业大学动物医学院, 兰州 730070
基金项目:“十二五”国家科技支撑计划重点课题(2012BAD12B03);中国农业科学院科技创新工程项目(CAAS-ASTIP-2014-LIHPS-03);甘肃省自然科学基金(1506RJYA146)
摘    要:为了探究黄藤素的体外细胞毒性,采用不同浓度的黄藤素作用山羊子宫内膜上皮细胞(EECs)后,采用MTT法绘制细胞生长曲线;倒置显微镜下观察细胞生长状态;瑞氏-姬姆萨染色法观察细胞形态学变化并通过测定乳酸脱氢酶(LDH)含量检测细胞膜的完整性。结果显示,当黄藤素的浓度为10~227 μg/mL时,黄藤素对EECs有促增殖的作用;当浓度大于227 μg/mL时出现细胞增殖抑制现象,药物对细胞的半数增殖抑制率(IC50)为360 μg/mL;瑞氏-姬姆萨染色结果显示,100、200 μg/mL的黄藤素作用于EECs时均未对其细胞核、细胞浆的形态产生影响;LDH结果显示,药物浓度≥250 μg/mL时细胞LDH的释放量极显著高于正常细胞组(P<0.01)。综上所述,黄藤素作用于山羊EECs后呈现一定的毒性作用,且呈现显著的剂量依赖效应,作用于EECs的安全剂量范围为≤250 μg/mL。

关 键 词:黄藤素  子宫内膜上皮细胞  细胞毒性  膜通透性  
收稿时间:2016-05-31

The Study on the Cytotoxicity of Palmatine on Goat Endometrial Epithelial Cells in vitro
YAN Bao-qi,DONG Shu-wei,WANG Dong-sheng,NA Li-dong,SANG Meng-qi,YANG Hong-zao,ZHANG Shi-dong,YAN Zuo-ting. The Study on the Cytotoxicity of Palmatine on Goat Endometrial Epithelial Cells in vitro[J]. China Animal Husbandry & Veterinary Medicine, 2016, 43(12): 3239-3244. DOI: 10.16431/j.cnki.1671-7236.2016.12.023
Authors:YAN Bao-qi  DONG Shu-wei  WANG Dong-sheng  NA Li-dong  SANG Meng-qi  YANG Hong-zao  ZHANG Shi-dong  YAN Zuo-ting
Affiliation:1. Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Animal Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China;
2. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China
Abstract:To explore the cytotoxicity of palmatine in vitro,goat endometrial epithelial cells (EECs) were stimulated by different concentrations of palmatine,then the growth curve of cell was drew by MTT assay which could detect the cell proliferation. The cell growth state was observed by inverted microscope,and Wright-Giemsa staining was used to observe morphological changes of the cells while LDH test was used to check the membrane permeability of EECs. The results showed that when the concentration was 10 to 227 μg/mL,palmatine had a role in promoting proliferation of the EECs,while when the concentration was more than 227 μg/mL,cell proliferation was inhibited,and 50% inhibitory rate of proliferation (IC50) of this drug on EECs was 360 μg/mL.Wright-Giemsa staining results showed that themorphology of the nucleus and cytolymph were not significantly changed when 100 and 200 μg/mL palmatine effected EECs. LDH results showed that when the palmatine concentration was more than 250 μg/mL,LDH content was extremely significantly higher than that of normal cell group(P<0.01).In conclusion,palmatine presented certain toxic effect on EECs with a significant dose-response effect,and the safe dose range of palmatine was less than 250 μg/mL.
Keywords:palmatine  endometrial epithelial cells  cytotoxicity  membrane permeability  
本文献已被 CNKI 等数据库收录!
点击此处可从《中国畜牧兽医》浏览原始摘要信息
点击此处可从《中国畜牧兽医》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号