| 猪接触传染性胸膜肺炎放线杆菌血清3型分子鉴定及药敏试验 |
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| 引用本文： | 李海利,徐引弟,宋毓民,朱文豪,张青娴,王克领,冯亚杰,候自花. 猪接触传染性胸膜肺炎放线杆菌血清3型分子鉴定及药敏试验[J]. 中国畜牧兽医, 2016, 43(1): 280-284. DOI: 10.16431/j.cnki.1671-7236.2016.01.041 |
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| 作者姓名： | 李海利 徐引弟 宋毓民 朱文豪 张青娴 王克领 冯亚杰 候自花 |
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| 作者单位： | 1. 河南省农业科学院畜牧兽医研究所, 郑州 450002;2. 山东省临沂市兰山区畜牧兽医局, 临沂 276000 |
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| 基金项目： | 河南省农业科学院优秀青年科技基金(2013YQ22);河南省农业科学院自主创新基金 |
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| 摘 要： | 为了解猪接触传染性胸膜肺炎放线杆菌(Actinobacillus pleuropneumoniae,APP)的血清型及耐药情况,本研究根据GenBank数据库设计1对引物,特异性的扩增950 bp核苷酸片段,对血清3型APP进行分子鉴定及药敏试验。结果显示,所得PCR产物经过测序,与GenBank已发表的血清3型APP的同源性达99%以上,所分离菌株耐药性较强,大多为多重耐药。通过对血清3型APP进行分子鉴定及药敏试验,为猪传染性胸膜肺炎的鉴定、诊断及防制提供了基础。
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| 关 键 词： | 猪传染性胸膜肺炎 胸膜肺炎放线杆菌 血清3型 药敏试验 |
| 收稿时间： | 2015-07-28 |
Molecular Identification and Antimicrobial Susceptibility Test of Porcine Contagious Actinobacillus pleuropneumoniae Serotype 3 |
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| LI Hai-li,XU Yin-di,SONG Yu-min,ZHU Wen-hao,ZHANG Qing-xian,WANG Ke-ling,FENG Ya-jie,HOU Zi-hua. Molecular Identification and Antimicrobial Susceptibility Test of Porcine Contagious Actinobacillus pleuropneumoniae Serotype 3[J]. China Animal Husbandry & Veterinary Medicine, 2016, 43(1): 280-284. DOI: 10.16431/j.cnki.1671-7236.2016.01.041 |
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| Authors: | LI Hai-li XU Yin-di SONG Yu-min ZHU Wen-hao ZHANG Qing-xian WANG Ke-ling FENG Ya-jie HOU Zi-hua |
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| Affiliation: | 1. Institute of Animal Husbandry and Veterinary Research, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China;2. Lanshan Bureau of Animal Husbandry and Veterinary, Linyi 276000, China |
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| Abstract: | In order to understand the serotype and drug resistance of porcine contagious Actinobacillus pleuropneumoniae(APP), a pair of primers was designed according to GenBank database to amplify specific 950 bp fragment, and the molecular identification and antimicrobial susceptibility test of APP serotype 3 were investigated.The results showed that the PCR product sequences were more than 99% homology with the APP serotype 3 published in GenBank.The isolated strains were highly resistant and multiple drug resistance.The molecular identification and antimicrobial susceptibility test of APP serotype 3 provided the basis for the identification, diagnosis and prevention of porcine contagious pleuropneumonia. |
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| Keywords: | porcine contagious pleuropneumonia Actinobacillus pleuropneumoniae serotype 3 antimicrobial susceptibility test |
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