猪传染性胸膜肺炎放线杆菌的分离鉴定及药敏试验

为了给天津某规模猪场提供猪传染性胸膜肺炎(PCP)的科学防控和合理的用药方案,本试验对该猪场疑似患PCP的病猪进行临床综合诊断和病理组织学观察,无菌采集病料,通过细菌的分离与培养、镜检、生化试验、卫星试验、PCR检测、动物致病性试验及药敏试验等实验室方法对分离菌进行系统分析。结果发现,病猪呼吸困难,病理剖检和病理组织学观察可见有典型的纤维素性肺炎变化;显微镜下分离菌呈多形性的革兰氏阴性杆菌,且其生化试验、卫星试验结果与传染性胸膜肺炎放线杆菌(APP)特性一致;用APP APX ⅣA毒素基因特异性引物扩增结果为阳性;该APP分离菌对小鼠具有致病性,且对头孢噻肟、氟苯尼考等药物高度敏感。根据实验室检测结果并结合临床综合诊断,最终判定该细菌分离株为猪传染性APP。

Isolation,Identification and Drug Sensitive Tests of Porcine Contagious Actinobacillus pleuropneumoniae

In order to provide a scientific prevention and treatment of porcine contagious pleuropneumia (PCP) disease in a pig farm of Tianjin,pigs with suspected PCP were carried on clinical comprehensive diagnosis and histopathological observations.A systematic analysis of isolated strain were conducted by laboratory diagnostic methods included bacterial isolation and culture,microscopic examination,biochemical tests,satellite phenomenon examination,PCR detection,animal pathogenicity experiments and drug sensitive tests.The most sick pigs were dyspnea,and the typical results of pathological anatomy and histopathological observations were fibrinous pneumonia.The gram-negative and polymorphous bacilli could be seen under the microscopy,its biochemical characteristics and satellite phenomenon were consistent with those of Actinobacillus pleuropneumoniae(APP).The amplified result of APP APX Ⅳ A gene was positive by specific primers.The isolated strain could be lethal to mice and it was highly sensitive to cefotaxime and florfenicol.Combined with the results of laboratory detection and the clinical comprehensive diagnosis,it was confirmed the isolated strain was APP.