| 流式细胞术细胞核分离缓冲液的改良及大、小果型荔枝幼果和果皮细胞分裂活性比较 |
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| 引用本文： | 李彩琴,王泽槐,徐咏珊,张劲霭,李建国. 流式细胞术细胞核分离缓冲液的改良及大、小果型荔枝幼果和果皮细胞分裂活性比较[J]. 园艺学报, 2011, 38(9): 1781-1785. DOI: 10.3969/j.issn.1000-4718.2011.09.023 |
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| 作者姓名： | 李彩琴 王泽槐 徐咏珊 张劲霭 李建国 |
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| 作者单位： | 华南农业大学中国荔枝研究中心,广州,510642 |
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| 基金项目： | 国家自然科学基金项目(30871694); 国家现代荔枝产业技术体系栽培生理岗位项目(nycytx-32-03); 教育部博士点基金项目(200805640003) |
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| 摘 要： | 以荔枝幼果为材料,比较5种常用细胞核分离缓冲液对制备样品DNA分辨率效果的影响,发现在Otto缓冲液基础上进行适当改进（增加还原剂种类和含量,把RNaseA直接加入缓冲液,降低离心力）,可获得最佳的提取效果,分辨率高,细胞G1/G0峰产生的变异系数低。通过比较具有相似遗传背景的‘妃子笑’早花大果和晚花小果以及具有不同遗传背景的大果（‘四两果’）和小果（‘陈紫’）型荔枝品种之间果实发育前期的幼果和果皮的细胞分裂活性发现,大果的细胞分裂活性高于小果,且持续时间长,说明果实大小差异与果实发育前期细胞分裂活性有关。
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| 关 键 词： | 荔枝 细胞分裂活性 流式细胞术 果实大小 核悬液 |
| 收稿时间： | 2011-01-10 |
| 修稿时间： | 2011-08-12 |
Improvement of the Cell Nuclei Suspensions for Flow Cytometry and Comparison on the Cell Division Activity of Litchi Fruitlet and Pericarp Which Having Different Final Fruit Size |
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| LI Cai-qin,WANG Ze-huai,XU Yong-shan,ZHANG Jin-ai, LI Jian-guo. Improvement of the Cell Nuclei Suspensions for Flow Cytometry and Comparison on the Cell Division Activity of Litchi Fruitlet and Pericarp Which Having Different Final Fruit Size[J]. Acta Horticulturae Sinica, 2011, 38(9): 1781-1785. DOI: 10.3969/j.issn.1000-4718.2011.09.023 |
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| Authors: | LI Cai-qin WANG Ze-huai XU Yong-shan ZHANG Jin-ai LI Jian-guo |
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| Affiliation: | 1. Department of Obstetrics and Gynecology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China;2. Department of Hepatology Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China. |
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| Abstract: | The effects of the five commonly used buffers on DNA nuclei isolation from fruitlet of litchi were evaluated.The results showed that improved method on Otto buffers(adding the content and type of reducing agent into Otto buffers,putting RNase A directly into the buffer and reducing the centrifugal force)could get the highest resolution and the lowest coefficient of variation G1/G0 peak of DNA nucleus,which was suitable for detecting the cell division activity of litchi fruitlet and pericarp.By comparing wit... |
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| Keywords: | litchi cell division activity flow cytometry fruit size nuclei suspension |
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