Hormonal replacement therapy in fish:human growth hormone gene function in hypophysectomized carp |
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Authors: | Zongbin Cuil Zuoyan Zhu |
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Institution: | (1) Chinese Academy of Sciences, Institute of Hydrobiology, Wuhan, Hubei, 430072, China;(2) Departments of Molecular and Cell Biology and Zoology, University of Aberdeen, Aberdeen, AB9 2TN, Scotland;(3) Departments of Molecular Biology and Zoology, University of Aberdeen, Aberdeen, AB9 2TN, Scotland |
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Abstract: | Transgenic common carp,Cyprinus carpio, produced by the microinjection of fertilized eggs with a linearized chimeric plasmid pMThGH, a human growth hormone (hGH)
gene with a mouse metallothionein-1 (MT) gene promoter in pBR322, were used to produce F1 and F2 transgenics. Following hypophysectomy of the transgenic F2 common carp, non-transgenic common carp and non-transgenic crucian carp, growth was monitored for up to 110 days. In addition,
recombinant hGH was injected subcutaenously into a group of the non-transgenic crucian carp. Growth rate analyses indicated
that (1) hypophysectomy of non-transgenic common carp and crucian carp results in the cessation of growth, (2) hGH administration
can stimulate the growth of hypophysectomized crucian carp and (3) hypophysectomized hGH-transgenic common carp continue to
grow in the absence of their own growth hormone, suggesting that the hGH-transgene is being expressed in tissues other than
the pituitary. |
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Keywords: | common carp crucian carp growth hormone growth hormone gene transgenic fish hormonal replacement hypophysectomy |
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