| 脱硫菌胞外聚合物提取及分泌量影响因素研究 |
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| 引用本文： | 高跃跃,张承中,葛洁. 脱硫菌胞外聚合物提取及分泌量影响因素研究[J]. 安徽农业科学, 2010, 38(19): 10233-10234,10398 |
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| 作者姓名： | 高跃跃 张承中 葛洁 |
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| 作者单位： | 西安建筑科技大学环境与市政工程学院,陕西西安,710055 |
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| 基金项目： | 国家自然科学基金，深圳市环境工程咨询中心资助项目 |
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| 摘 要： | [目的]选择一种较优的黄单胞杆菌EPS提取方法,并分析环境因素对EPS分泌量的影响。[方法]分别采用离心、加热、超声、硫酸、EDTA和甲醛加碱法提取EPS,测定提取物中多糖、蛋白质及DNA含量,以评价提取效率及对细胞的破坏程度。利用正交试验设计考察温度、培养基pH值等因素对EPS分泌量的影响。[结果]甲醛加碱法和超声法的提取效率较高。通过对不同超声功率的比较,选择在50W下超声4min提取EPS。正交试验结果表明,黄单胞杆菌EPS最大分泌量的环境条件为pH值6.0,温度32℃,菌液比7∶100,金属盐溶液投加量3ml。[结论]黄单胞杆菌EPS提取采用超声法;环境因素中温度对分泌量的影响最大。
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| 关 键 词： | 胞外聚合物 提取方法 正交设计 影响因素 |
Study on the Extraction of Extracellular Polymeric Substances from Desulfurization Bacterium and the Influencing Factors of Its Yield |
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| GAO Yue-yue et al. Study on the Extraction of Extracellular Polymeric Substances from Desulfurization Bacterium and the Influencing Factors of Its Yield[J]. Journal of Anhui Agricultural Sciences, 2010, 38(19): 10233-10234,10398 |
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| Authors: | GAO Yue-yue et al |
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| Affiliation: | GAO Yue-yue et al(School of Environmental and Municipal Engineering,Xi'an University of Architecture and Technology,Xi'an,Shaanxi 710055) |
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| Abstract: | [Objective ] The aim was to compare different mathods of extracting EPS from xanthomonas and study the effect of environmental factors on the yield of EPS.[Method]Six methods including centrifugation,heating,sonication,sulfuric acid,EDTA,formaldehyde plus sodium hydroxide were adopted to extract EPS,and then the extraction abilities of these methods were evaluated in terms of extraction efficiency and cells destruction through measuring concentrations of polysaccharide,protein and DNA respectively.The ortho... |
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| Keywords: | Extracellular polymeric substances Extraction method Orthogonal design Influence factors |
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