| 大豆愈伤组织继代培养的最佳条件研究 |
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| 引用本文： | 张郁松,寇炜材. 大豆愈伤组织继代培养的最佳条件研究[J]. 大豆科学, 2007, 26(2): 190-193 |
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| 作者姓名： | 张郁松 寇炜材 |
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| 作者单位： | 西安武警工程学院,西安,710086 |
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| 摘 要： | 探讨基本培养基、培养条件、植物生长调节剂等因素对大豆继代培养的影响.大豆愈伤组织继代培养的最佳条件:以MS BA0.5 mg/L 2,4-D2.0 mg/L为培养基,pH在5.8～6.0之间,在1 000～1 500 Lx,2 h/d光照条件下,培养21 d后生长较好,愈伤组织生长量和增长倍数可分别达到11.23 g/瓶和10.23.
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| 关 键 词： | 大豆 愈伤组织 继代培养 |
| 文章编号： | 1000-9841(2007)02-0190-04 |
| 修稿时间： | 2006-07-03 |
RESEARCH ON THE OPTIMUM CONDITIONS FOR SOYBEAN CALLUS SUBCULTURE |
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| ZHANG Yu-song,KOU Wei-cai. RESEARCH ON THE OPTIMUM CONDITIONS FOR SOYBEAN CALLUS SUBCULTURE[J]. Soybean Science, 2007, 26(2): 190-193 |
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| Authors: | ZHANG Yu-song KOU Wei-cai |
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| Abstract: | In this paper,the subculture of soybean were studied from the aspects of basic media,culturing conditions,phytohormones etc.The optimum conditions for soybean callus subculture were as follows: Growth status of callus was better on MS medium supplemented with BA0.5 mg/L,2,4-D2.0 mg/L,the callus yield and increasing times were the highest,it can reached 11.23 g/Flask and 10.23.The suitable pH value of medium was among 5.8~6.0.The callus cultured in light(1000~1500Lx,12 h/d) for 21d were suitable for the callus growth. |
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| Keywords: | Soybean Callus Subculture |
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