| HPLC法测定不同品种鸡蛋中的胆固醇含量 |
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| 引用本文： | 杨琴,戴瑞彤. HPLC法测定不同品种鸡蛋中的胆固醇含量[J]. 农产品加工．学刊, 2006, 0(8): 71-74 |
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| 作者姓名： | 杨琴 戴瑞彤 |
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| 作者单位： | 中国农业大学,食品科学与营养工程学院,北京,100083 |
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| 摘 要： | 采用高效液相色谱法测定鸡蛋中的胆固醇含量,以C18(5μm,250mm×4.6mm)为固定相,乙腈-异丙醇(4∶1)为流动相,流速为0.5mL/min,检测波长为210nm,测定结果是,胆固醇浓度在0.1￣0.8g/L范围内与色谱峰面积呈良好的线性关系。结果表明,不同鸡蛋品种中胆固醇含量差异较大。该方法快速,灵敏,简便易行,无须皂化便可直接定量。
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| 关 键 词： | 胆固醇 高效液相色谱 蛋黄 |
| 文章编号： | 1671-9646（2006）08-0071-04 |
| 收稿时间： | 2006-08-01 |
| 修稿时间： | 2006-08-01 |
Determination of Cholesterol Content in Different Egg Products by HPLC Method |
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| Yang Qin,Dai Ruitong. Determination of Cholesterol Content in Different Egg Products by HPLC Method[J]. Nongchanpin Jlagong.Xuekan, 2006, 0(8): 71-74 |
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| Authors: | Yang Qin Dai Ruitong |
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| Abstract: | A reversed-phase high performance liquid chromatographic assay was applied for the rapid determination of cholesterol content in egg yolk. The C18 column(5 μm,250 mm×4.6 mm)was selected and the mobile phase consisted of acetonitrile and 2-propanol(4∶1)at a flow rate of 0.5 mL/min. The detection wavelength was 210 nm. A linearity was obtained from 0.1 to 0.8 g/L of cholesterol with a good correlation coefficient. Omission of saponification has made this method more convenient,and can be used in yolk cholesterol assay. |
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| Keywords: | cholesterol HPLC yolk |
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