应用酶联免疫分析方法检测镇江市内水域水样和底泥中的邻苯二甲酸二乙酯

应用邻苯二甲酸二乙酯(Diethyl phthalate,DEP)抗体,建立了间接竞争性ELISA方法,并对反应条件进行优化,进而测定了镇江市内水域中DEP含量。结果显示优化后的ELISA方法检测范围为20~320 ng·m L~(-1),最低检测限为8.2 ng·m L~(-1)。方法特异性高,与其他邻苯二甲酸酯类似物几乎无交叉反应。ELISA方法批内差在3.38%~9.09%,批间差在3.76%~12.78%。检测方法检测水样和底泥样本中DEP含量,添加回收率在90.46%~122.75%。镇江市内古运河和运粮河水样和底泥样本中DEP的检出率分别为62.5%和87.5%,其中水样中DEP含量最高可为6.61 ng·m L~(-1),底泥样本中DEP含量最高为86.9 ng·g~(-1)。

Development of a enzyme-linked immunosorbent assay for diethyl phthalate analysis in water and sediment samples from rivers in Zhenjiang City

Based on a polyclonal antibody targeting diethyl phthalate(DEP), a sensitive indirect competitive enzyme-linked immunosorbent assay(ELISA) was proposed and several parameters were investigated. Under the optimized conditions, the developed method showed high sensitivity to DEP(the limit of detection, 8.2 ng·mL^-1) and high selectivity with cross-reactivities less than 4.29% to seven structurally related phthalate esters. The linear range was from 20 ng·mL^-1 to 320 ng·mL^-1. The recoveries(90.46%~122.75%) and reproducibility(CV of intra-assay,3.38%~9.09%; CV of inter-assay, 3.76%~12.78%) were satisfactory. Our proposed ELISA indicated that it had good agreement with those given by GC-MS, which was applied to detect DEP in waters and sediments from ancient canal and Yunliang River. And DEP was found in most of target areas(the detection rate, 62.5% in waters and 87.5% in sediments), the concentration was up to 6.61 ng·mL^-1 and 86.9 ng·g^-1 in water and sediments, respectively.