| SSR和AFLP分子标记鉴定巴西橡胶树种质资源的比较研究 |
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| 引用本文： | 钟淦彬,李维国,位明明,郑学项,易晓洁. SSR和AFLP分子标记鉴定巴西橡胶树种质资源的比较研究[J]. 热带作物学报, 2016, 37(10): 1855-1862. DOI: 10.3969/j.issn.1000-2561.2016.10.002 |
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| 作者姓名： | 钟淦彬 李维国 位明明 郑学项 易晓洁 |
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| 作者单位： | 1. 中国热带农业科学院橡胶研究所,海南儋州 571737;自贡市职业培训学院,四川自贡643000;2. 中国热带农业科学院橡胶研究所,海南儋州,571737;3. 海南乐东县乐东中学,海南乐东,572500;4. 自贡职业技术学校,四川自贡,643000 |
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| 基金项目： | “十一五”国家科技支撑计划（No. 2006BAD01A06~3）;植物新品种DUS测试指南研制项目。 |
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| 摘 要： | 选用178份巴西橡胶树种质材料,采用SSR和AFLP分子标记技术对巴西橡胶树种质资源进行鉴定,探讨2种方法的应用效果并加以比较分析。结果显示:利用多态性强的19对SSR引物,检测到92个等位基因;用25对AFLP引物组,检测到702条有多态性的带。SSR位点的平均多态性信息量(PIC)值为0.46,而AFLP多态性带比例是54.88%,AFLP比SSR分子标记具有更高的多态性。结果说明,AFLP标记比SSR标记更适合于巴西橡胶树种质材料的鉴定和保护,同时进一步验证了在作物遗传资源的研究中,采用多种分子标记方法可以获得更加准确的研究数据。
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| 关 键 词： | 巴西橡胶树 SSR AFLP 分析比较 |
Identification for Rubber Tree(Hevea brasiliensis)Genetic Resources Using SSR and AFLP Markers |
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| ZHONG Ganbin,LI Weiguo,WEI Mingming,ZHENG Xuexiang and YI Xiaojie. Identification for Rubber Tree(Hevea brasiliensis)Genetic Resources Using SSR and AFLP Markers[J]. Chinese Journal of Tropical Crops, 2016, 37(10): 1855-1862. DOI: 10.3969/j.issn.1000-2561.2016.10.002 |
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| Authors: | ZHONG Ganbin LI Weiguo WEI Mingming ZHENG Xuexiang YI Xiaojie |
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| Affiliation: | 1 Rubber Research Institute, CATAS 2 Zigong Vocational Training Institute;Rubber Research Institute, CATAS;Rubber Research Institute, CATAS;The middle school of Ledong County,;Zigong Vocational Technical School, Zigong |
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| Abstract: | The 178 rubber tree germplasm resources were identified using SSR and AFLP markers and to explore the application effects of two methods by comparing the result of two markers. The results showed that 19 pairs of SSR primers, total 92 polymorphic fragments were detected; 25 AFLP primer combinations(Ecor I/Mse I)were used, and total 701 polymorphic bands were produced. AFLP was higher polymorphic than SSR. The average of polymorphic information content(PIC)was 0.46 for SSRs. Percentage of polymorphic AFLP bands was 54.88%. According to the comparative result acquired in this study, AFLP is more suitable for rubber tree germplasm materials identification and protection than SSR. Meanwhile further validated that in crop genetic resources research, use more kinds of molecular marker method can obtain more accurate data. |
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| Keywords: | Hevea brasiliensis SSR AFLP Comparative analysis |
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