高效液相色谱法测定秸秆浸提液或腐解液中12种酚酸

建立了同时测定秸秆浸提液或腐解液中12种酚酸物质(没食子酸、原儿茶酸、龙胆酸、对羟基苯甲酸、绿原酸、香草酸、咖啡酸、丁香酸、对香豆酸、阿魏酸、芥子酸、水杨酸)的反相高效液相色谱测定方法。采用Waters HLB(500 mg、60μm、6 m L)固相萃取小柱对水溶液中的酚酸物质进行浓缩,以含0.5%乙酸的乙腈和0.5%乙酸的水溶液为流动相进行梯度洗脱,选用二极管阵列检测器,30 min内可对12种酚酸物质同时检测,各种酚酸均可达到基线分离。加标回收试验表明,样品中12种酚酸类化合物的加标回收率为79.41%~101.92%,12种酚酸保留时间和峰面积的RSD分别在0.02%~0.1%和0.25%~2.26%之间。该方法快速、灵敏、准确,适用于同时测定秸秆浸提液、腐解液、土壤溶液及水溶液中12种酚酸物质含量。

Determination of 12 phenolic acids in straw extract or straw decomposed liquid through high performance liquid chromatography

A method of reversed phase high performance liquid chromatography was established, which could be used to determine the con-tents of 12 phenolic acids(gallic acid, protocatechuic acid, gentisic acid, p-hydroxybenzoic acid, chlorogenic acid, vanilla acid, coffee acid, syringic acid, p-coumaric acid, ferulic acid, sinapic acid, salicylic acid)simultaneously in straw extract or decomposed liquid. Waters HLB (500 mg, 60μm, 6 mL)solid-phase extraction cartridges were used to concentrate phenolic acids in aqueous solution. The gradient elution was carried out using acetonitrile solution containing 0.5%acetic acid and 0.5%acetic acid solution as the mobile phase. The diode array detector was used for detection, and the determination of 12 kinds of phenolic acids could be completed simultaneously in 30 minutes. All of phenolic acids could reach the baseline separation. The recovery rate of 12 phenolic acids were between 79.41%~101.92%, and the RSD of retention time and peak area of 12 phenolic acids were between 0.02%~0.1%and 0.25%~2.26%, respectively. This method is rapid, sensi-tive and accurate, suitable for the simultaneous determination of 12 phenolic acids existed in straw extract, straw decomposed liquid, soil so-lution and aqueous solution.