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褪绿叶斑病毒(CLSV)对苹果属植物过氧化物酶活性及同工酶的影响
引用本文:肖艳,成明昊.褪绿叶斑病毒(CLSV)对苹果属植物过氧化物酶活性及同工酶的影响[J].西南农业大学学报,1992,14(2):100-105.
作者姓名:肖艳  成明昊
作者单位:西南农业大学园艺系 (肖艳,成明昊),西南农业大学园艺系(李育农)
基金项目:四川省科委生物技术项目
摘    要:26个苹果属植物种类受苹果褪绿叶斑病毒(CLSV)侵染后,其过氧化物酶活性升高,嫁接后95天内出现1或2个增酶高峰.同工酶的谱带也增多,特别是B 区变化更为明显,但不同种类反应不一,楸子、酸苹果等对CLSV 抗性强的种类出现2个增酶高峰,且表现时间早,峰值低,同工酶出现2次特异性谱带;冬白果等对CLSV 敏感的种类则只有1个增酶高峰,表现时间迟,峰值高,只出现一次特异性谱带。这种过氧化物酶活性及其同工酶谱的变化可作为鉴定苹果属植物对CLSV 的抗性的生化指标.

关 键 词:苹果属  褪绿叶斑病毒  过氧化物酶

EFFECTS OF CLSV ON THE CHANGES IN PEROXIDASE ACTIVITY AND IN THE ISOZYMOGRAMS OF Malus SPECIES
Xiao Yan Cheng Minghao Li Yunong.EFFECTS OF CLSV ON THE CHANGES IN PEROXIDASE ACTIVITY AND IN THE ISOZYMOGRAMS OF Malus SPECIES[J].Journal of Southwest Agricultural University,1992,14(2):100-105.
Authors:Xiao Yan Cheng Minghao Li Yunong
Abstract:Twenty-six species of the genus Malus were grafted on M.toringgoides seedling stockswhich were inoculated with CLSV so as to study the changes in their peroxidase activityand isozyme zymograms.All the grafters showed an hincrease in their peroxidase activity,and one or two peaks appeared about 95 days after grafting.Some new specific bands wereobserved in their isozyme zymograms,especially in area B.However,the species in thisstudy differed greatly in these changes.Such a method proved to be rapid and effective inassessing the CLSV resistance and sensitivity in the genus of Malus.
Keywords:malus  rootstock  chlorosis spot  peroxidase  isoenzymes
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