Comparison Between Elisa and Dye Test for Detection of Naturally Acquired Toxoplasma Gondii Antibodies in the Goat |
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| Authors: | Bjø rn P. Berdal,Ø rjan Olsvik,Torbjø rn Alrnlid,Hans Jø rgen Larsen,Anne-Marie Lorentzen-Styr |
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| Affiliation: | Norwegian Defence Microbiological Laboratory, National Inisitute of Public Healh, Department of Microbiology and Immunology, Norwegian College of Veterinary Medicine, and the Department of Animal Breeding, Agricultural University of Norway, Geitmyrsveien 75, Oslo 4, Norway |
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| Abstract: | Toxoplasma gondii IgG antibodies were measured in 212 goat sera, comparing the Sabin-Feldman dye-test and a three-layer sandwich enzyme-linked immunosorbent assay (ELISA). With 98 % concordance obtained between these 2 tests, the results are at the same paragon as for human sera. Accordingly, the ELISA sandwich procedure appears to be suitable for large-scale analysis of goat sera. The discordant 2 % were ELISA positive and dye-test negative. One possible explanation of the divergent titres is given using an immunized goat model.Key words: Toxoplasma gondii, goat, antibodies, dye-test, ELISA |
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