| 壳寡糖诱导烟草防御酶系活性变化及PR-1a基因表达研究 |
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| 引用本文： | 商文静,吴云锋,赵小明,杜昱光,商鸿生.壳寡糖诱导烟草防御酶系活性变化及PR-1a基因表达研究[J].植物病理学报,2010,40(1):99-102. |
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| 作者姓名： | 商文静 吴云锋 赵小明 杜昱光 商鸿生 |
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| 作者单位： | 1 西北农林科技大学植物保护学院 陕西省农业分子生物学重点实验室, 杨凌 712100;2 中国科学院大连化学物理研究所, 大连 116023 |
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| 基金项目： | 教育部长江学者和创新团队发展计划，高等学校学科创新引智计划，西北农林科技大学博士启动基金 |
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| 摘 要： | The activity change of defensive enzymes and PR-1a gene expression of tobacco (Nicotiana tabacum) seedling induced by chito-oligosaccharides were studied. The results showed that high level systemic acquired resistance (SAR) was expressed in tobacco plants treated with chito-oligosaccharides solution at the concentration of 50 μg/mL. PAL activity increased greatly with 2 peaks, the activity of SOD decreased initially followed by an increase with higher increment, and the activity of POD peaked early followed by a gentle fall in chito-oligosaccharide treated plants. The PR-1a gene was strongly expressed in tobacco due to systemic acquired resistance induced by chito-oligosaccharides. At 168 h after inoculation the expression quantity (co-pies/2 μL) of PR-1a gene was increased to 2 469.6 in treated tobacco leaf, reached 392.6% than that at 0 h after inoculation, it was increased 3.05 times of that in untreated control.
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| 关 键 词： | 系统获得抗病性 诱导 烟草叶片 基因序列 壳寡糖 酶活性 活性测定 防御酶系活性 系统抗病性 |
Changes of defensive enzymes and PR-1a gene expression of tobacco induced by chito-oligosaccharides |
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| SHANG Wen-jing,WU Yun-feng,ZHAO Xiao-ming,DU Yu-guang,SHANG Hong-sheng.Changes of defensive enzymes and PR-1a gene expression of tobacco induced by chito-oligosaccharides[J].Acta Phytopathologica Sinica,2010,40(1):99-102. |
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| Authors: | SHANG Wen-jing WU Yun-feng ZHAO Xiao-ming DU Yu-guang SHANG Hong-sheng |
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| Institution: | 1 College of Plant Protection, Northwest A & F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, China;2 Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China |
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| Abstract: | The activity change of defensive enzymes and PR-l a gene expression of tobacco(Nicotiana tabacum)seedling induced by chaito-oligosaccharides were studied.The results showed that high level systemic acquired resistance(SAR)was expressed in tobacco plants treated with chito-oligosaccharides solution at the concentration of 50 μg/mL.PAL activity increased greatly with 2 peaks,the activity of SOD decreased initially followed by an increase with higher increment,and the activity of POD peaked early followed by a gentle fall in chito-oligosaccharide treated plants.The PR-la gene was strongly expressed in tobacco due to systemic acquired resistance induced by chito-oligosaccharides.At 168 h after inoculation the expression quantity(copies/2μL)of PR-la gene was increased to 2 469.6 in treated tobacco leaf,reached 392.6%than that at 0 h after inoculation,it Was increased 3.05 times of that in untreated control. |
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| Keywords: | chito-oligosaccharides SAR defensive enzyme PR-1a gene |
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