Association of bovine papillomavirus type 2 (BPV-2) and urinary bladder tumours in cattle from the Azores archipelago |
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| Authors: | Ana R Resendes Sante Roperto Francesca Trapani Chiara Urraro Armindo Rodrigues Franco Roperto Giuseppe Borzacchiello |
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| Institution: | a Center for Research in Natural Resources (CIRN), University of the Azores, Apt 1422, 9501-855 Ponta Delgada, Portugal;b Department of Pathology and Animal Health, Faculty of Veterinary Medicine, Naples University Federico II, Via F. Delpino, 1-80137 Naples, Italy |
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| Abstract: | Urinary bladder tumours in cattle are caused by chronic ingestion of bracken fern and BPV-1/2 infection. The objective of the present study was to assess if BPV-2 was present in urinary bladder lesions from cattle with chronic enzootic haematuria (CEH) from the Azores archipelago (Portugal), in order to gain further information regarding the epidemiologic distribution of this virus. Samples were analysed using PCR specific primers for BPV-2 DNA and an immunohistochemistry for BPV E5 oncoprotein detection. We found a 28% incidence rate of BPV-2 DNA in different types of tumours and cystitis cases (13 out of 46 samples). Tested positive samples for PCR were also positive for the viral E5 oncoprotein; protein immunolabeling was mainly detected within the cytoplasm of urothelial cells, displaying a juxtanuclear distribution. This is the first report of BPV-2 detection in urinary bladder tumours associated with CEH in cattle from the Azores archipelago. |
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| Keywords: | BPV-2 E5 oncoprotein PCR Cattle Bracken fern Chronic enzootic haematuria |
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