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| 重庆彭水野生美味牛肝菌菌丝分离与鉴定 | |
| 引用本文： | 王正春,蒋海艳,杨笑笑,李月文,李继晖,郑凌凌.重庆彭水野生美味牛肝菌菌丝分离与鉴定[J].中国食用菌,2012,31(1):46-48. |
| 作者姓名： | 王正春 蒋海艳 杨笑笑 李月文 李继晖 郑凌凌 |
| 作者单位： | 1. 重庆市林业科学研究院,重庆 400036;西南大学资源环境学院,重庆 400715 2. 重庆市林业科学研究院,重庆,400036 3. 西南大学资源环境学院,重庆,400715 4. 彭水自治县林业科技推广站,四川 彭水,409600 |
| 基金项目： | 重庆市科技攻关项目“彭水县野生牛肝菌人工驯化关键技术研究”（CSTC,2010AC1111）;“武陵山区食用菌良种高效栽培试验示范”（CSTC,2010AB1047）;重庆市林科院科技计划项目“万州区老林村林下食用菌栽培示范”、“重庆桉树、竹林林下种植模式技术研究”. |
| 摘 要： | 通过2种不同的培养基对彭水2种野生牛肝菌子实体进行分离、培养,同时,进行液体培养,培养的菌丝经过ITS鉴定,放入-80℃超低温冰箱中保藏.结果表明,2种牛肝菌菌丝均从组织块表面边缘生长,菌丝洁白、小,不向周围培养基生长,老化速度较快,老化过程中菌丝减少,最后变为固体状物,并且分泌褐色水珠；液体培养菌丝体生长缓慢,培养时间较长,菌丝球小、少；菌丝球经过鉴定后,确定2种牛肝菌均为美味牛肝菌. |
| 关 键 词： | 重庆 彭水 美味牛肝菌 分离培养 液体培养 ITS鉴定 保藏 |
Hyphe Separation and Identification of Wild Boletus edulis in Chongqing Pengshui |
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| WANG Zheng-chun , JIANG Hai-yan , YANG Xiao-xiao , LI Yue-wen , LI Ji-hui , ZHENG Ling-ling.Hyphe Separation and Identification of Wild Boletus edulis in Chongqing Pengshui[J].Edible Fungi of China,2012,31(1):46-48. | |
| Authors: | WANG Zheng-chun JIANG Hai-yan YANG Xiao-xiao LI Yue-wen LI Ji-hui ZHENG Ling-ling |
| Institution: | 3 (1.Forestry Research Institute of Chongqing, Chongqin 400036; 2.Southwest University, Chongqin 400715; 3.Pengshui Autonomous County Forestry Science and Technology Promotion Station, Pengshui Sichuan 409600) |
| Abstract: | Through two different culture medium, two wild Boletus edulis were seperated and cultured, and training hyphae were identificated by ITS, which were stord in the refrigerator of -80℃. The results showed that the two Boletus edulis hyphe liver tissue were growth from mass surface edge, and the hyphe were white and small. The hyphe didn＇t grow around the culture medium, and aging faster. The mycelia by liquid culture grew slowly, and the ball was small. Hyphae ball by determining were Boletus edulis. |
| Keywords: | Chongqing Pengshui Boletus edulis Separation training Liquid culture ITS identification Preservation |
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