北虫草菌种人工分离的研究

随着生产规模的不断扩大,北虫草的培育也暴露了一个问题,正常菌种经多次转管继代和冰箱久存之后,会出现不产子实体现象,这些问题严重干扰了虫草的生产。为此,进行了有关北虫草不同部位组织分离和继代培养的试验,并筛选出性状优良的菌株。结果表明,子实体靠近顶端分离的菌种易于萌发,孢子分离得到的菌株性状更好,北虫草子实体本身就具有抑菌作用,因此,在选取表面消毒方式时,一定要适宜,不可时间过长,以免对子实体造成伤害,不产生菌丝。

Research on Different Parts of the Cordyceps military Tissue Isolation and Continuous Culture

With the procreative scale dilating constantly, the culture of Cordyceps sinensis had some questions, when the normal bacterium was transferred many times or be kept in the fridge for a long time, it maybe didn＇t procluce me ：rucnfication. So an experiment on the Cordyceps military tissue isolation and continuous culture were done, and a strong spices were got. The results showed that the part near top was more likely to sprout. North Cordyceps was some kind of bacteriostat. The one which isolated from spore was the best. It must be sterilized properly, or it won＇t produce mycelium.