| 马尾松银松素合酶基因启动子区的克隆及特征分析 |
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| 引用本文： | 王冰梅,;郭晋隆,;叶冰莹,;许莉萍,;陈由强.马尾松银松素合酶基因启动子区的克隆及特征分析[J].甘蔗(福建),2008(4):292-296. |
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| 作者姓名： | 王冰梅 ;郭晋隆 ;叶冰莹 ;许莉萍 ;陈由强 |
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| 作者单位： | [1]福建师范大学生命科学学院,福建福州350108; [2]农业部甘蔗遗传改良重点开放实验室,福建福州350002; [3]福建师范大学发育与神经生物学福建省高等学校重点实验室,福建福州350108 |
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| 基金项目： | 国家自然科学基金（30371177）,福建省教育厅基金（JA07045）资助项目. |
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| 摘 要： | 银松素合酶（pinosylvin synthase,PS）是合成银松素类植物抗毒素的关键酶。本文根据前期工作所获得的PS基因序列,设计基因特异引物,利用连接介导的染色体步行技术,从马尾松总基因组中扩增PS基因上游的启动子区;通过PLAN-CARE等分析软件对5′侧翼区近700 bp进行启动子特征分析,预测启动子区调控元件。结果表明,在翻译起始密码子上游167 nt处可能存在TATA-box。此外还发现3个GATA-box（-260 nt、-295 nt、-386 nt和-295 nt）和若干个TGAC-like序列。
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| 关 键 词： | 银松素合酶 基因克隆 染色体步行 启动子分析 |
Molecular cloning and analysis of pinosylvin synthase gene promoter from Pinus massoniana |
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| Institution: | WANG Bing-mei, GUO Jin-long, YE Bing-ying, XU Li-ping, CHEN You-qiang(1. College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350108, China; 2. Key Laboratory of Sugarcane Genetic Improvement, Ministry of Agriculture, Fuzhou, Fujian 350002, China; 3. State Key Laboratory of Developmental Biology and Neurobiology, Fujian Normal University, Fuzhou, Fujian 350108, China) |
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| Abstract: | Pinosylvin synthase （PS）, is a key enzyme in the biosynthesis of pinosylvin-type phytoalexins. In the study, PS gene specific primers were designed, and the upstream sequence of PS gene was cloned by genomic walking based on ligation-mediated PCR method. The regulatory element of gene promoter was analyzed by PLANCARE on line. This sequence was about 700 bp in length, containing one TATA-box, three GATA-boxes, and several TGAC-like sequences. |
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| Keywords: | pinosylvin synthase gene cloning genomic walking promoter analysis |
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