| 辣椒疫霉作用下叶绿素a/b结合蛋白的表达 |
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| 引用本文： | 林明,;贺俐,;徐波,;官德义,;牟少亮,;何水林. 辣椒疫霉作用下叶绿素a/b结合蛋白的表达[J]. 甘蔗(福建), 2008, 0(4): 297-301 |
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| 作者姓名： | 林明, 贺俐, 徐波, 官德义, 牟少亮, 何水林 |
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| 作者单位： | [1]福建农林大学生命科学学院,福建福州350002; [2]福建农林大学作物科学学院,福建福州350002; [3]井冈山大学生命科学学院,江西吉安343009 |
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| 基金项目： | 国家自然科学基金资助项目（30571128）. |
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| 摘 要： | 从基于cDNA-AFLP分析的辣椒应答疫霉侵染的表达谱中找到1个特异表达基因的序列;利用这个序列设计引物,采用基于PCR技术的96孔文库筛选方法,从疫霉处理的辣椒cDNA文库中筛选出这个应答辣椒疫霉侵染的候选基因的全长cDNA;经生物信息学分析,推测这个长度为1022 bp的候选基因为辣椒叶绿素a/b结合蛋白（chlorophyll a/b binding protein,cab）基因;并利用荧光定量PCR法检测cab在疫霉侵染下的表达情况。
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| 关 键 词： | 辣椒 辣椒疫霉 CDNA-AFLP 叶绿素a/b结合蛋白 实时荧光定量PCR |
Expression of the chlorophyll a/b binding protein infected by Phytophthora capsici in pepper |
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| Affiliation: | LIN Ming , HE Li, XU Bo , GUAN De-yi, MOU Shao-liang , HE Shui-lin ( 1. College of Life Science ; 2. College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; 3. College of Life Science, Jinggangshan University, Ji＇an, Jiangxi 343009, China) |
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| Abstract: | A sequence of specific expression gene was found in the expression profile responding to infected Phytophthora capsici in popper based on eDNA-FALP analysis, and the sequence was used to design the primer, one positive candidate resistant gene eDNA was isolated from the pepper eDNA library by PCR-based 96-hole screening. According to the results of bioinformafies analysis, it was estimated that the candidate resistant gene with the length of 1022 bp was chlorophyll a/b binding protein gene. |
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| Keywords: | pepper Phytophthora capsici cDNA-AFLP chlorophyll a/b binding protein real time RT-PCR |
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