| ELISA与HPLC-MS对饲用玉米中2种霉菌毒素的比较分析 |
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| 引用本文： | 钟兴文,王炳彦,陈琛,杨旭艳,杨秀娟,陈莹,孙照程,华雪妃,孔凡虎,章雨竹,陶琳丽.ELISA与HPLC-MS对饲用玉米中2种霉菌毒素的比较分析[J].中国饲料,2019(17):98-103. |
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| 作者姓名： | 钟兴文 王炳彦 陈琛 杨旭艳 杨秀娟 陈莹 孙照程 华雪妃 孔凡虎 章雨竹 陶琳丽 |
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| 作者单位： | 云南农业大学动物科学技术学院;牟定县新桥镇畜牧兽医站;大理白族自治州动物卫生监督所 |
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| 基金项目： | 云南省重大科技专项“高原优质肉鸡产业工程化技术研发集成与示范推广”(2016ZA008);云南省现代农业禽蛋产业技术体系项目(2017KJTX0017) |
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| 摘 要： | 实验采集饲用玉米样品共30份,通过酶联免疫法(ELISA)和高效液相色谱-串联质谱联用(HPLC-MS)法对玉米赤霉烯酮(ZEA)和T-2毒素(T-2)2种霉菌毒素进行检测,旨在比较两种方法对饲用玉米中霉菌毒素的检测效果。结果表明:两种检测方法对饲用玉米中的ZEA均100%检出。检测T-2时ELISA法的检出率为83.33%,HPLC-MS法则未检出。两种方法检测出的霉菌毒素含量均都表现出ELISA法的检出值普遍高于HPLC-MS法。ELISA法前处理简单、检出速度较快且适合大批量样品检测,但因干扰因素较多易出现假阳性。HPLC-MS法前处理复杂、成本昂贵、操作要求高,但检测结果较为稳定和准确。说明在实际生产应用中,应结合两种检测方法的优缺点,用ELISA法初筛,检测结果呈阳性后,再用HPLC-MS法进行精确定量,这样既能实现检测目的又能节省检测费用。
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| 关 键 词： | 酶联免疫法 高效液相色谱-串联质谱联用 饲用玉米 玉米赤霉烯酮 T-2毒素 |
Comparative analysis of two kinds of mycotoxins in forage corn by ELISA and HPLC-MS |
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| ZHONG Xingwen,WANG Bingyan,CHEN Chen,YANG Xuyan,YANG Xiujuan,CHEN Ying,SUN Zhaocheng,HUA Xuefei,KONG Fanhu,ZHANG Yuzhu,TAO Linli.Comparative analysis of two kinds of mycotoxins in forage corn by ELISA and HPLC-MS[J].China Feed,2019(17):98-103. |
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| Authors: | ZHONG Xingwen WANG Bingyan CHEN Chen YANG Xuyan YANG Xiujuan CHEN Ying SUN Zhaocheng HUA Xuefei KONG Fanhu ZHANG Yuzhu TAO Linli |
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| Institution: | (Faculty of Animal Science and Technology,Yunnan Key Laboratory of Animal Nutrition and Feed Science,Yunnan Agricultural University,Kunming,Yunnan Province 650201,China;Animal Husbandry and Veterinary Station of XinqiaoTown in Mouding County Chuxiong Prefecture of Yunnan province,Chuxiong,Yunnan Province 675501,China;Animal Health Supervision Institute of Dali Bai Autonomous Prefecture,Dali,Yunnan Province 671000,China) |
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| Abstract: | ZHONG Xingwen;WANG Bingyan;CHEN Chen;YANG Xuyan;YANG Xiujuan;CHEN Ying;SUN Zhaocheng;HUA Xuefei;KONG Fanhu;ZHANG Yuzhu;TAO Linli(Faculty of Animal Science and Technology,Yunnan Key Laboratory of Animal Nutrition and Feed Science,Yunnan Agricultural University,Kunming,Yunnan Province 650201,China;Animal Husbandry and Veterinary Station of XinqiaoTown in Mouding County Chuxiong Prefecture of Yunnan province,Chuxiong,Yunnan Province 675501,China;Animal Health Supervision Institute of Dali Bai Autonomous Prefecture,Dali,Yunnan Province 671000,China) |
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| Keywords: | ELISA HPLC-MS forage maize zearalenone trichothecenes |
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