Transmission efficiency of three isolates of maize stripe tenuivirus in relation to virus titre in the planthopper vector

Isolates of maize stripe tenuivirus (MSt V) from Florida (US), Costa Rica (CR), and Nigeria, Africa (AF), were transmitted to maize plants by the planthopper Peregrinus maidis (from Hawaii) with respective frequencies of 0,18, and 60% after a 1-day acquisition access period on diseased plants, and with frequencies of 18, 71 and 93%, respectively, after a 7-day access period. These isolates were transmitted transovarially to progeny planthoppers with respective frequencies of 21, 32, and 47%. The latent period in the vector, following oral acquisition of MSt V, was significantly longer in the US isolate than in the AF-or CR isolates. ELISA tests of MSt V-inoculative planthoppers indicated a significantly lower titre of MSt V-US compared with MSt V-CR or MSt V-AF. These results suggest that, compared with the US isolate, the AF and CR isolates of MSt V multiply faster and reach higher levels in, and are transmitted more efficiently by, P. maidis from Hawaii.