高效液相色谱荧光法测定辣椒酱中的罗丹明 B

目的]建立辣椒酱中罗丹明B的高效液相色谱荧光检测法。方法]将样品中的罗丹明B用一定比例的丙酮和正己烷提取，经过氧化铝固相萃取柱净化浓缩后，用液相色谱荧光检测器检测其含量。结果]试验得出，罗丹明B在0．005—0．050mg／kg浓度范围内，线性关系良好，相关系数为0．999；方法的定量检测限为0．005mg／kg，平均回收率高。结论]该试验建立的方法快速、准确、灵敏度高，适用于辣椒酱中罗丹明B的测定。

Determination of Rhodamine B in Chili Patse by HPLC Fluorimetric Method

Objective ] The aim was to establish a HPLC fluorimetric method for the determination of Rhodamine B in chili parse. Method ] Rhodamine B was extracted with acetone/hexane from chili patse samples. After concentrated and purified by alumina cartridge, the Rhodamine B content in the chili patse was determined by using high performance liquid chromatography with fluorescence detector. Result ] Within the concentration range of 0.005 - O. 050 mg/kg, the peak area of Rhodamine B presents good linear relation with the concentration with the related coefficient of 0. 999. The detection limit of the method is 0.005 mg/kg and with high average recovery rate. Conclusion] It is a fast and accu- rate method to detect Rhodamine B in chili patse with high sensitivity.