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| 水稻核糖体蛋白（OsRPL14）基因的克隆及表达分析 | |
| 引用本文： | 杜志如,席 江,万 佳,江 斌,徐永菊,张 琦,徐正君. 水稻核糖体蛋白（OsRPL14）基因的克隆及表达分析[J]. 中国农学通报, 2008, 24(4): 130-134 |
| 作者姓名： | 杜志如 席 江 万 佳 江 斌 徐永菊 张 琦 徐正君 |
| 作者单位： | 1. 四川农业大学水稻研究所,温江,611130 2. 四川农业大学水稻研究所,温江,611130;四川农业大学,作物基因资源与遗传改良教育部重点实验室,雅安,625014 |
| 摘 要： | 通过荧光差异显示(fluorescent differential display，FDD)以及RT-PCR技术，得到一个水稻核糖体蛋白基因。该基因的cDNA全长为565 bp，编码一个具有134个氨基酸残基的多肽，推测分子量是15.4 kD。与其他植物中核糖体蛋白L14的相似性为82.84%～88.06%，命名该基因为OsRPL14。在低温（8℃）及干旱条件处理下，该基因表达量在根和茎中都有比较明显的增加趋势。推测该基因在逆境反应中起着重要作用。 |
| 关 键 词： | 安徽省 安徽省 菜地土壤 重金属 |
| 收稿时间： | 2008-02-03 |
| 修稿时间： | 2008-02-03 |
Cloning and Expression Analysis of a Ribosomal Protein from Rice(OsRPL14) |
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| Du Zhiru,Xi Jiang,Wan Ji,Jiang Bin,Xu Yongju,Zhang Qi,Xu Zhengjun. Cloning and Expression Analysis of a Ribosomal Protein from Rice(OsRPL14)[J]. Chinese Agricultural Science Bulletin, 2008, 24(4): 130-134 | |
| Authors: | Du Zhiru Xi Jiang Wan Ji Jiang Bin Xu Yongju Zhang Qi Xu Zhengjun |
| Affiliation: | Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, China; Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Ya’an 625014 |
| Abstract: | Using a fluorescent differential display method, a gene was isolated from rice seedling. The full length cDNA was 565 bp and encoded a 134 amino acid(15.4kD). The results of similar analysis indicated that OsRPL14 had 82.84%-88.06% identities with other ribosomal proteins, named OsRPL14. The level of the gene expression was enhanced in response to cold(8℃)and dry. We suggest the gene might play important roles in stress respones. |
| Keywords: | ricezz ribosomal proteinzz cloningzz expression zz |
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