|
|||||
|
|
| 磁珠富集法开发毛竹SSR标记引物 | |
| 引用本文： | 彭镇华,刘贯水,李潞滨.磁珠富集法开发毛竹SSR标记引物[J].林业科学研究,2011,24(6):743-748. |
| 作者姓名： | 彭镇华 刘贯水 李潞滨 |
| 作者单位： | 1. 国际竹藤网络中心,北京100102;中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京100091 2. 国际竹藤网络中心,北京,100102 3. 中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京100091 |
| 基金项目： | 林业公益性行业科研专项(No. 200704001);国家林业局重点项目(No.2007-01);"十一·五"科技支撑(2006BAD19B08) |
| 摘 要： | 用磁珠富集法筛选毛竹基因组DNA的SSR分子标记,利用磁珠法对毛竹基因组DNA的AFLP片段进行了富集,分别构建了富含GT、AG、CCA重复基元的富集文库,利用克隆PCR法对文库进行筛选,检测菌落数分别为1080、620、630个,阳性菌落中含目的重复的序列分别为137、73、41个,富集效率分别为12.7％、11.8... |
| 关 键 词： | 毛竹 磁珠富集法 文库筛选 SSR引物 |
| 收稿时间： | 2010/6/30 0:00:00 |
Development of Polymorphic Simple Sequence Repeats Markers in Phyllostachys edulis by Magnesphere |
|
| PENG Zhen-hu,LIU Guan-shui and LI Lu-bin.Development of Polymorphic Simple Sequence Repeats Markers in Phyllostachys edulis by Magnesphere[J].Forest Research,2011,24(6):743-748. | |
| Authors: | PENG Zhen-hu LIU Guan-shui and LI Lu-bin |
| Institution: | International Centre for Bamboo and Rattan, Beijing 100102, China;Research Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China;International Centre for Bamboo and Rattan, Beijing 100102, China;Research Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China |
| Abstract: | The objective of this work is to seek SSR markers for Phyllostachys edulis. Magnesphere method was used to conentrate SSR containing sequences from Ph. edulis Genomic DNA AFLP fragments. Three SSR-enriched libraries (GT, AG, and CCA) were constructed. The Clone-PCR method was used to screen positive clones, 1 080, 620, 630 clones were screened in GT, AG, CCA libraries, and 137, 73, 41 SSR-containing sequences were obtained, at concentration rate of 12.7%, 11.8%, 6.5% respectively. The result showed that the concentration rate of dinucleotide repeat libraries is higher than trinucleotide libraries. After sequences analyzing, 53 pairs of SSR primers were designed, 31 of which amplified the objective fragment in Ph. edulis at the rate of 58.9%. |
| Keywords: | Phyllostachys edulis Magnesphere Method Libraries Screening SSR Primers |
| 本文献已被 万方数据 等数据库收录！ | |
| 点击此处可从《林业科学研究》浏览原始摘要信息 | |
| 点击此处可从《林业科学研究》下载免费的PDF全文 | |